OT-I cells were purified on a Histopaque gradient post activation in-vitro or eluted from a CD8-negative selection MACS column (Miltenyi) from single cell suspensions of splenocytes. Once purified, the cells were stained (CellTrace™ Violet/ CellTrace™ CFSE) diluted and transferred i.v into sex matched C57BL/6 recipients unless otherwise indicated. For radiation-attenuated sporozoite (RAS) immunization strategies, 2×104 naïve cells were transferred 24 hours prior to RAS delivery. For intravital imaging and lymphocyte tracking experiments, 5×106 cells were transferred to each mouse. For naïve and activated co-transfer experiments, approximately 2.5×106 cells of each type were transferred. For protection assays, 2×106 cells were transferred 4 hours prior to infection with P. berghei.
Cd8 negative selection macs column
Manufactured by Miltenyi Biotec
The CD8-negative selection MACS column is a laboratory equipment used for the isolation and enrichment of CD8-negative cells from a heterogeneous cell population. The column selectively retains CD8-positive cells, allowing the CD8-negative cells to pass through, enabling their isolation and collection for further analysis or experimental purposes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cd8 negative selection macs column
1
Adoptive Transfer of Labeled T Cells
2
In vivo Tracking of Activated T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
OT-I cells were purified on a Histopaque gradient post activation in-vitro or eluted from a CD8-negative selection MACS column (Miltenyi) from single cell suspensions of splenocytes. Once purified, the cells were stained (CellTrace™ Violet/ CellTrace™ CFSE) diluted and transferred i.v into sex matched C57BL/6 recipients unless otherwise indicated.
For radiation-attenuated sporozoite (RAS) immunisation strategies, 2x10 4 naïve cells were transferred 24 hours prior to RAS delivery. For intravital imaging and lymphocyte tracking experiments, 5x10 6 cells were transferred to each mouse. For naïve and activated co-transfer experiments, approximately 2.5x10 6 cells of each type were transferred. For protection assays, 2x10 6 cells were transferred 4 hours prior to infection with P.berghei.
For radiation-attenuated sporozoite (RAS) immunisation strategies, 2x10 4 naïve cells were transferred 24 hours prior to RAS delivery. For intravital imaging and lymphocyte tracking experiments, 5x10 6 cells were transferred to each mouse. For naïve and activated co-transfer experiments, approximately 2.5x10 6 cells of each type were transferred. For protection assays, 2x10 6 cells were transferred 4 hours prior to infection with P.berghei.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!