Dextran

Dextran and Ficoll were purchased from VWR International (Radnor, PA, USA). Catalase, cytochrome C, H 2 O 2 , PMA, SOD, Triton X-100, 4-ABAH, DPI, L-GSH, NAC, uric acid, and DAPI were obtained from Sigma (St. Louis, MO, USA). Amplex UltraRed, APF, CellEvent Caspase-3/7 Detection Reagent Kit, CM-H 2 DCF-DA, DHR123, PrestoBlue resazurin, goat anti-rabbit Alexa Fluor 488, and Sytox Green and Orange were obtained from Thermo Fisher Scientific Life Sciences (Waltham, MA, USA). The polyclonal goat anti-human neutrophil elastase and rabbit anti-citrullinated histone H3 antibodies were purchased from Abcam (Cambridge, United Kingdom). Protein block was obtained from Dako (Carpinteria, CA, USA), and fixation buffer and antihuman IL-8 ELISA were purchased from BioLegend (San Diego, CA, USA). Tryptone soya broth was purchased from Oxoid (Thermo Scientific, Hampshire, United Kingdom), and Vectashield containing DAPI was obtained from Vector Laboratories (Burlingame, CA, USA). Tissue-culture plastic was obtained from Becton Dickinson (Franklin Lakes, NJ, USA), Nunc (Roskilde, Denmark), and Sarstedt (N ümbrecht, Germany).