Enzcheck ser thr phosphatase assay kit

Tail DNA was extracted with DirectPCR Lysis Reagent (Viagenbiotech). Lipopolysaccharides from Escherichia coli 055:B5 (#L4005), actinomycin D (#A9415) were purchased from Sigma. Thioglycollate (#236310) was purchased from Becton Dickinson. Taqman and SYBR Green PCR assay kits, GAPDH, MIP1α, MIP-2, CXCL9, CXCL11 and PP2ACα primer/probes were purchased from Applied Biosystems. Mouse TNF-α, IL-6, KC, IL-10, IFNα4 and IFNβ quantitet primers were purchased from Qiagen. Phospho-p38, -p44/42 MAPK, -SAPK/JNK, -c-Jun, -IκBα,-IKKα/β, -NFκB p65, -stat3, -IRF7/IRF3 and non-phospho-IκB antibodies were obtained from Cell Signaling. Anti-PP2A antibody (1D6, #05–421) was purchased from Millipore. Anti-mouse IFNAR-1 antibody (MAR1–5A3) neutralization antibody was purchased from Biolegend. SB 203580 (p38 inhibitor), JNK inhibitor II, Wedelolactone (IKK inhibitor II), FR180204 (ERK inhibitor II) were purchased from Calbiochem. EnzCheck Ser/Thr phosphatase assay kit (#R33700) was purchased from Molecular Probes. Crosslink Magnetic IP/Co-IP kit (#88805) was purchased from Pierce. Mouse TNF-α, IL-10 ELISA Kits were purchased from Invitrogen. Mouse IFN-α ELISA kit was purchased from Life Technologies. A 6-plex kit [IL-1β, IL-10, IL-6, IL-12(p40), IFN-γ, KC and TNF-α] was customized by Bio-Rad.