Log In Sign up
The largest database of trusted experimental protocols

Mouse anti wt1

Manufactured by Santa Cruz Biotechnology
Visit Supplier
Sourced in China

The Mouse anti-WT1 is a primary antibody that recognizes the Wilms' Tumor 1 (WT1) protein, a transcription factor involved in the regulation of gene expression. This antibody is suitable for use in various immunoassay techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to detect and analyze the expression of WT1 in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Mouse anti zo 1, by Thermo Fisher Scientific (1 mentions) Rabbit anti six2, by Proteintech (1 mentions) Mouse anti e cadherin, by BD (1 mentions) Goat anti synaptopodin, by Santa Cruz Biotechnology (1 mentions) Axio vert a1 microscope, by Zeiss (1 mentions) Calcitriol, by Merck Group (1 mentions) Rabbit anti vdr, by Santa Cruz Biotechnology (1 mentions) Rabbit anti nephrin, by Abcam (1 mentions) Rabbit anti gapdh, by Abcam (1 mentions)

4 protocols using mouse anti wt1

1

Immunofluorescence Imaging with Custom Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
We conducted confocal imaging on a Zeiss Pascal 5 confocal laser microscope. All secondary antibodies were purchased from Jackson labs. Previously published primary antibodies include rabbit anti-ZHX3 (4457–2B5)6 (link),7 (link), mouse anti-FLAG tag antibody, mouse anti-HSV tag antibody, and rat anti-mouse APA monoclonal antibodies ASD37 and ASD4119 (link)–21 (link). We immunized host animals with recombinant peptides to raise the following custom polyclonal antibodies (Proteintech Laboratories Chicago, immunogen and antibody name/s notated): Rabbit anti-mouse ZHX2 (amino acids 15 to 150; 6965–1B3, 6965–2B4), Sheep anti-rat ZHX1 (amino acids 820 to 873; 8161-C2), Sheep anti-rat ZHX1 (amino acids 16 to 307; 8161-N5); Rabbit antirat APA (amino acids 44 to 942; 4091–2F), Rabbit anti-rat EPHRIN B1 (amino acids 25 to 239; 8605-R2B6), Guinea Pig anti-mouse WT1 (amino acids 1 to 271 of mouse glomerular WT1; 6786–49F). The following primary antibodies were purchased: mouse anti-WT1 (Santa Cruz # sc-7385); guinea pig anti-Nephrin (Fitzgerald # 20R-NP002); Sheep anti-VWF (Fitzgerald # 20R-VS001); Mouse anti-ZHX2 (Abnova, Taiwan # H00022882-A01; used for Western blot and confocal imaging), Rabbit anti-ZHX3 (Santa Cruz # sc-292339).
Macé C., Avila M.D., Marshall C.B., Kharlyngdoh J., Das R., Molina-Jijon E., Blazquez H.D., Shastry S., Soria E., Wetzels J., Dijkman H., Avila-Casado C., Clement L.C, & Chugh S.S. (2019). The zinc fingers and homeoboxes 2 protein ZHX2 and its interacting proteins regulate upstream pathways in podocyte diseases. Kidney international, 97(4), 753-764.
+ Open protocol
+ Expand
2

Immunodetection of Kidney Progenitors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies used include rabbit anti-Six2 (Proteintech, 11,562-1-AP, 1:1000), rabbit anti-Six1 (Cell Signaling, #12,891, 1:500), guinea pig anti-cytokeratin 8 + 18 (Abcam, AB194130, 1:500 and Progen, GP-KP, 1:500), mouse anti-zo-1 (Invitrogen, 339,100, 1:400), mouse anti-E-cadherin (BD biosciences, 618082, 1:400), mouse anti-WT1 (Santa Cruz, sc-7385, 1:50) JAG1 (Santa Cruz, sc-8303, 1:100).
Schuh M.P., Yarlagadda S., Alkhudairy L., Preusse K, & Kopan R. (2023). Characterizing post-branching nephrogenesis in the neonatal rabbit. Scientific Reports, 13, 19234.
+ Open protocol
+ Expand
3

Quantifying Kidney Glomerular Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
After antigen retrieval and blocking, 5-mm kidney paraffin sections were incubated overnight at 4 °C with goat anti-synaptopodin (Santa Cruz Biotechnology), mouse anti-WT-1 (Santa Cruz Biotechnology), mouse anti-p16INK4a (Abcam), or mouse anti-C/EBPα primary antibodies (Santa Cruz Biotechnology). After washing, the sections were incubated with FITC-conjugated donkey anti-mouse/anti-goat secondary antibodies. The sections were examined using an AxioVert A1 microscope (Zeiss, Germany) with a digital camera. ImageJ 1.51k software (National Institutes of Health) was used to measure the level of immunostaining in the glomeruli. First, the images were converted into 8-bit grayscale images. The glomerular regions were selected to measure the area. After grayscale inversion, the optical density (OD) was calibrated. Then, the positively stained regions in the glomeruli were selected with appropriate settings and concordant thresholds, the cells were counted, the positively stained area was calculated, and both measurements were normalized to the glomerular areas.
Zhang L., Zhou F., Yu X., Zhu Y., Zhou Y., Liu J., Liu Y., Ma Q., Zhang Y., Wang W, & Chen N. (2019). C/EBPα deficiency in podocytes aggravates podocyte senescence and kidney injury in aging mice. Cell Death & Disease, 10(10), 684.
+ Open protocol
+ Expand
4

Nephrin, VDR, and WT1 Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Calcitriol was purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Primary antibodies used in this study were rabbit anti-nephrin (Abcam Ltd. Shanghai, China, ab136894), rabbit anti-VDR (Santa Cruz Biotechnology, Inc. sc1008), mouse anti-WT1 (Santa Cruz Biotechnology, Inc. sc393498), rabbit anti-GAPDH (Abcam Ltd. Hong Kong, China, ab9485). Anti-mouse secondary antibodies (115-035-003) and anti-rabbit secondary antibodies (111-035-003) were purchased from Jackson ImmunoResearch Laboratories Inc (West Grove, PA, USA).
Shi W., Guo L., Liu G., Peng T., Li H., Xie T., Li D., Zhen J., Wang Y., Yang H, & Yang X. (2018). Protective effect of calcitriol on podocytes in spontaneously hypertensive rat. Journal of the Chinese Medical Association : JCMA, 81(8).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!