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Mir 124 3p

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MiR-124-3p is a microRNA that functions as a regulator of gene expression. It is involved in various biological processes and is commonly studied in the context of cellular development and differentiation.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Itgb1, by Thermo Fisher Scientific (1 mentions)

2 protocols using mir 124 3p

1

qRT-PCR expression analysis of miR-124-3p and integrins

Cited 2 times
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TaqMan qRT-PCR probes and primers were obtained from Thermo Fisher Scientific (Waltham, MA, USA) as follows: miR-124-3p (product ID: 000446; Thermo), ITGA3 (product ID: Hs01076879_m1), ITGB1 (product ID: Hs01127536_m1) and ITGA2 (product ID: Hs00158127_m1). GUSB (product ID: Hs99999908_m1) and RNU48 (product ID: 001006) were used as internal controls. The procedure for qRT-PCR was described in previous studies [30 (link), 41 (link)].
Idichi T., Seki N., Kurahara H., Fukuhisa H., Toda H., Shimonosono M., Yamada Y., Arai T., Kita Y., Kijima Y., Mataki Y., Maemura K, & Natsugoe S. (2018). Involvement of anti-tumor miR-124-3p and its targets in the pathogenesis of pancreatic ductal adenocarcinoma: direct regulation of ITGA3 and ITGB1 by miR-124-3p. Oncotarget, 9(48), 28849-28865.
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2

Transfection of miRNA and siRNA in PDAC

Cited 1 time
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Pre-miR miRNA precursors and siRNAs were purchased from Thermo Fisher Scientific, as follows: miR-124-3p (product ID: PM 10245), negative control miRNA (product ID: AM 17111), two ITGA3 siRNAs (product IDs: HSS105529 and HSS179967), two ITGB1 siRNAs (product IDs: HSS105559 and HSS105561), and negative control siRNA (product ID: D-001810-10). Lipofectamine RNAiMAX and Lipofectamine 2000 (Thermo Fisher Scientific) were used for transfection into PDAC cell lines. The transfection efficiencies of miRNA in cancer cells were calculated as described in previous studies [42 (link)].
Idichi T., Seki N., Kurahara H., Fukuhisa H., Toda H., Shimonosono M., Yamada Y., Arai T., Kita Y., Kijima Y., Mataki Y., Maemura K, & Natsugoe S. (2018). Involvement of anti-tumor miR-124-3p and its targets in the pathogenesis of pancreatic ductal adenocarcinoma: direct regulation of ITGA3 and ITGB1 by miR-124-3p. Oncotarget, 9(48), 28849-28865.
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