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Anti mouse pd l1 clone 10f 9g2

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The Anti-mouse PD-L1 (clone 10F-9G2) is a laboratory reagent that binds to the murine programmed death-ligand 1 (PD-L1) protein. This antibody can be used for detection and analysis of PD-L1 expression on cells.

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Lab products found in correlation

Anti mouse cd45 clone 30 f11, by BioLegend (2 mentions) Recombinant mouse ifn γ, by Thermo Fisher Scientific (1 mentions) Anti mouse cd31 clone mec 13, by BD (1 mentions) Anti mouse cd8 clone 53 6, by Thermo Fisher Scientific (1 mentions) Anti foxp3 antibody clone fjk 16s, by Thermo Fisher Scientific (1 mentions) Anti mouse cd25 pc61, by Thermo Fisher Scientific (1 mentions) Anti mouse cd4 clone rm4 5, by BD (1 mentions) Facscanto 2, by BD (1 mentions) Fixation permeabilization solution, by BD (1 mentions) Perm wash, by BD (1 mentions) Anti mouse cd80 clone 16 10a1, by BioLegend (1 mentions) Anti mouse f4 80 clone bm8, by BioLegend (1 mentions) Zombie violet fixable viability kit, by BioLegend (1 mentions) Anti mouse human cd11b clone m1 70, by BioLegend (1 mentions) Anti mouse cd86 clone gl 1, by BioLegend (1 mentions) 7 aminoactinomycin d 7 aad, by Thermo Fisher Scientific (1 mentions) Sytox blue, by Thermo Fisher Scientific (1 mentions) Anti mouse mhcii clone m5 114, by BioLegend (1 mentions) Clone m a712, by BD (1 mentions) Antimouse gr1 clone rb6 8c5, by BioLegend (1 mentions)

4 protocols using anti mouse pd l1 clone 10f 9g2

1

HPV 16 E7 Peptides and Flow Cytometry Analysis

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HPV 16 E7aa49–57 peptide, RAHYNIVTF, and HPV 16 E7aa43–62 peptide, GQAEPDRAHYNIVTFCCKCD, were synthesized by GenScript (Piscataway, NJ). FITC-conjugated anti-mouse CD4 (clone RM4–5), FITC- and PerCP-conjugated anti-mouse CD8a (clone 53.6.7), APC-conjugated anti-mouse CD3 (clone 17A2), and PE- and APC-conjugated anti-mouse CD45 (clone 30-F11) antibodies were purchased from BD Pharmingen (San Diego, CA). FITC-conjugated anti-mouse Gr-1 (clone RB6–8C5), PE-conjugated anti-mouse CD11b (clone M1/70), anti-Foxp3 (clone FJK-16s) were purchased from eBioscience (San Diego, CA). FITC-conjugated anti-gp38, APC-conjugated anti-mouse PD-1 (clone RMP1–30), and anti-mouse PD-L1 (clone 10F-9G2) antibodies were purchased from Biolegend (San Diego, CA). PE-conjugated, HPV 16 E7aa49–57 peptide-loaded H-2Db tetramers were purchased from MBL International (Japan). Recombinant mouse IFN-γ was purchased from eBioscience. Purified anti-mouse PD-1 monoclonal antibodies (clone 29F.1A12) were purchased from Bio X Cell (West Lebanon, NH).
Peng S., Tan M., Li Y.D., Cheng M.A., Farmer E., Ferrall L., Gaillard S., Roden R.B., Hung C.F, & Wu T.C. (2020). PD-1 Blockade Synergizes with Intratumoral Vaccination of a Therapeutic HPV Protein Vaccine and Elicits Regression of Tumor in a Preclinical Model. Cancer immunology, immunotherapy : CII, 70(4), 1049-1062.
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2

Mouse Tumor Single-Cell Immune Profiling

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Preparation of single-cell suspension of mouse tumor tissues by mechanical grinding. All single-cell suspensions were incubated with rat anti-mouse CD16/CD32 blocking antibody (4 μg/ml) for 15 min after thorough filtration and precipitation, stained with fluorescein-conjugated antibodies, multiple washed with PBS, and resuspended in 7-AAD (exclude non-viable cells). For anti-mouse CD4 (Clone RM4-5, BD Biosciences), anti-mouse CD8 (clone 53-6.7, eBioscience), anti-mouse CD45 (clone 30-F11, Biolegend), anti-mouse CD25 (PC61.5, eBioscience), anti-mouse PD-L1 (clone 10 F.9G2, BioLegend) and anti-mouse CD31 (clone MEC 13.3, BD Biosciences) staining, after incubation for 1 h, cells were washed with PBS for three times (1500 rpm, 5 min each), then detected by flow cytometry (BD FACSCanto II). For FoxP3 staining, after incubation, cells were washed and fixed with 1 ml of fixation & permeabilization solution (BD Biosciences) for 30–60 min, and washed twice with Perm Wash (BD Biosciences). Intracellular staining with anti-FoxP3 antibody (clone FJK-16s, eBioscience) was performed for 1 h. The next steps are the same as described above.
Liu S., Qin T., Liu Z., Wang J., Jia Y., Feng Y., Gao Y, & Li K. (2020). anlotinib alters tumor immune microenvironment by downregulating PD-L1 expression on vascular endothelial cells. Cell Death & Disease, 11(5), 309.
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3

Comprehensive Immune Cell Profiling

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Anti-human CD47 (clone B6H12, BD Biosciences), anti-mouse F4/80 (clone BM8, BioLegend), anti-Sirpα (clone P84, BioLegend), anti-mouse/human CD11b (clone M1/70, BioLegend), anti-mouse CD45 (clone 30-F11, BioLegend), anti-mouse MHC II (clone M5/114.15.2, BioLegend), anti-mouse CD206 (clone C068C2, BioLegend), anti-mouse CD80 (clone 16-10A1, BioLegend), anti-mouse CD86 (clone GL-1, BioLegend), anti-mouse PD-L1 (clone 10F.9G2, BioLegend), anti-mouse Gr1(clone RB6-8C5,BioLegend), anti-human CD14 (clone HCD14, BioLegend), and anti-human CD71 (clone CY1G4, BioLegend; clone OKT9, ThermoFisher; clone L01.1 and clone M-A712, BD Biosciences) were used for FACS analyses. Antibodies were Phycoerythrin (PE)-, PE/Cyanine7, APC, APC/Cyanine7, PerCP/Cyanine5.5, PE/Dazzle™ 594, Alexa Flour® 700 or BV605 conjugated, or fluorophore-conjugated secondary antibodies were used. Annexin V (BD Biosciences), Sytox blue (ThermoFisher), 7-Aminoactinomycin D (7-AAD, ThermoFisher), or Zombie Violet™ Fixable Viability Kit (Biolegend) was used to exclude dead cells. Flow cytometry was performed using the BD LSRFortessa cell analyzers (BD).
Chen J., Cao X., Li B., Zhao Z., Chen S., Lai S.W., Muend S.A., Nossa G.K., Wang L., Guo W., Ye J., Lee P.P, & Feng M. (2021). Warburg Effect Is a Cancer Immune Evasion Mechanism Against Macrophage Immunosurveillance. Frontiers in Immunology, 11, 621757.
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4

Immunohistochemical Analysis of PD-L1

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Optimum cutting temperature-embedded tumors were sectioned (Histoserv, Germantown, MD). Sections of 6-μM thickness were fixed via immersion in ice-cold acetone before serum block and staining with anti-mouse PD-L1 (clone 10F.9G2) or isotype control antibody (BioLegend). Signal was amplified with ABC peroxidase and slides were stained with diaminobenzidine (Vector Labs, Burlingame, CA) per protocol. Stained samples were washed before nuclear counterstaining with Hematoxylin QS (Vector Laboratories) and mounted with Permount (Fisher Scientific). Whole slides were digitized for analysis using the Aperio ScanScope CS system. Eight, 40× high-powered fields were analyzed from each sample with exclusion of necrotic regions.
Shah S., Caruso A., Cash H., Van Waes C, & Allen C.T. (2016). Pools of programmed death-ligand within the oral cavity tumor microenvironment: Variable alteration by targeted therapies. Head & neck, 38(8), 1176-1186.
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