Torin 1 s2827

Manufactured by Selleck Chemicals

Torin 1 (S2827) is a small-molecule inhibitor that specifically targets the mTOR kinase, a key regulator of cell growth and metabolism. It is commonly used in scientific research to investigate the role of the mTOR pathway in various biological processes.

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Lab products found in correlation

Bovine serum albumin (bsa), by Sangon (1 mentions) Sc 47778, by Santa Cruz Biotechnology (1 mentions) A600669, by Sangon (1 mentions) Bay 11 7 082 hy 13453, by MedChemExpress (1 mentions) Rapamycin s1039, by Selleck Chemicals (1 mentions) Cycloheximide c7698, by Merck Group (1 mentions) Rapamycin r8781, by Merck Group (1 mentions) Phenformin p7045, by Merck Group (1 mentions)

Spelling variants of this product

S2827 (3 citations) Torin (2 citations)

2 protocols using torin 1 s2827

Antibodies and Pharmacological Inhibitors

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The antibodies against phosphorylated p-rpS6 (4858), rpS6 (2217), MTOR (2972), (p)-MTOR (5536), RELA (8242), p-RELA (4812) were sourced from Cell Signaling Technology. The antibody against MMP12 (ab52897) was acquired from Abcam, and the one against ACTB (sc-47778) was obtained from Santa Cruz Biotechnology. All these antibodies were diluted in accordance with the manufacturer’s instructions using either 5% nonfat dried milk (Sangon Biotech, A600669) or 5% BSA (Sangon Biotech). For flow cytometry, we used anti-mouse CD45 PE-Cyanine7 (25-0451-82), anti-mouse CD11c+ FITC (11-0114-82), anti-human/mouse phospho-MTOR (S2448) eFluor 450 (48-9718-42), and anti-human/mouse phosphor-rpS6 (S235/S236) APC (17-9007-42) antibodies from eBioscience. The anti-mouse Siglec-F PE (562068) antibody was purchased from BD Biosciences. BAY 11-7082 (HY-13453) was obtained from Medchem Express and was used at a final concentration of 2.5 μM in the culture medium. Rapamycin (S1039) and Torin 1 (S2827) were sourced from Selleck, and their final concentrations were 1.25 and 125 nM, respectively.

Dong L., Wang Y., Chen H., Li Z., Xu X., Zhou J., Shen H, & Chen Z. (2024). MTOR Suppresses Cigarette Smoke-Induced Airway Inflammation and MMP12 Expression in Macrophage in Chronic Obstructive Pulmonary Disease. International Journal of Chronic Obstructive Pulmonary Disease, 19, 269-279.

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Validated Pharmacological Modulators

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Rapamycin (R8781), AMP (A1752), ATP (A2383), phenformin (P7045), 2-deoxy-D-glucose (2DG; D6134), H2O2 (18304) and cycloheximide (C7698) were from Sigma-Aldrich. Torin1 (S2827) was from Selleckchem and A769662 (ab120335) was from Abcam.

Smiles W.J., Ovens A.J., Yu D., Ling N.X., Morrison K.R., Hoque A., Scott J.W., Galić S., Langendorf C.G., Kemp B.E., Peterson J., & Oakhill J.S. (2021). Novel torin1-sensitive phosphorylation sites on the metabolic regulator AMPK revealed by label-free mass spectrometry.

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