Cytospins of cells from bone marrow or peripheral blood were fixed for 2 minutes in methanol, air-dried, and stained for 20 minutes with filtered modified solution of Giemsa stain (Sigma Aldrich, Cat # 48900, St Louis, MO), diluted (1:20) with buffer solution pH6.5, rinsed with distilled water, air-dried and examined using low and high magnifications with a Leica DMR microscope (Leica Microsystems, Wetzlar GmbH, Germany) connected to Nuance multispectral imaging system FX using Nuance version 3.0.2 software (PerknElmer, Inc., Hopkinton, MA)
Modified solution of giemsa stain
Manufactured by Merck Group
Modified solution of Giemsa stain is a laboratory reagent commonly used in microscopy for the staining and differentiation of cellular components. It provides a consistent and reliable staining method for the visualization of various cell types and structures.
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Lab products found in correlation
2 protocols using modified solution of giemsa stain
1
Cytospin and Giemsa Staining Protocol
2
Cytospin and Giemsa Staining Protocol
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