RNA was extracted from ES cells, lung tissue, or lung tumors with the RNA-BEE reagent according to the manufacture's protocol (Fisher Scientific). 0.5 - 2 μg RNA was reverse transcribed using Omniscript RT kit (QIAGEN) and Oligo dT (QIAGEN) as primers. Resultant cDNA was used as a template to amplify targets of interest using the primers listed in Supplementary Table 4 .
Rna bee reagent
Manufactured by Thermo Fisher Scientific
RNA-BEE reagent is a specialized solution designed for the isolation and purification of RNA from various biological samples. It is a key component in the RNA extraction process, providing a reliable and efficient method to obtain high-quality RNA for downstream applications.
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Lab products found in correlation
4 protocols using rna bee reagent
1
RNA Extraction and RT-PCR Analysis
2
RNA Extraction and RT-PCR Analysis
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RNA was extracted from ES cells, lung tissue, or lung tumors with the RNA-BEE reagent according to the manufacture's protocol (Fisher Scientific). 0.5 - 2 μg RNA was reverse transcribed using Omniscript RT kit (QIAGEN) and Oligo dT (QIAGEN) as primers. Resultant cDNA was used as a template to amplify targets of interest using the primers listed in Supplementary Table 4 .
3
RNA Extraction Using RNA-Bee Reagent
4
RNA Extraction and Reverse Transcription
Check if the same lab product or an alternative is used in the 5 most similar protocols
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RNA was extracted from cells with the RNA-BEE reagent according to the manufacture’s protocol (Fisher Scientific). 0.5–2.0 μg of RNA was reverse transcribed using Omniscript RT kit (QIAGEN) with an Oligo dT (QIAGEN) primer. Resultant cDNA was used as a template to amplify targets of interest using the primers (P1-P6) listed in S1 Table S1 Fig .
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