Rabbit anti ampkα2

Manufactured by Cell Signaling Technology

Rabbit anti-AMPKα2 is a primary antibody that specifically recognizes the AMPKα2 subunit of the AMP-activated protein kinase (AMPK) enzyme. AMPK is a cellular energy sensor that plays a crucial role in regulating metabolism and energy homeostasis.

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AMPKα2 (15 citations) Anti-AMPKα2 (4 citations)

2 protocols using rabbit anti ampkα2

AMPK Purification and Phosphorylation

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Recombinant His-tagged AMPK complexes were expressed in Escherichia coli and purified by chromatography on nickel-Sepharose [9 (link)]. AMPK was phosphorylated on Thr¹⁷² by overnight incubation with MgATP and CaMKKβ, and repurified as previously described [9 (link)]. Subsequent incubation with MgATP and CaMKKβ did not increase Thr¹⁷² phosphorylation, demonstrating that Thr¹⁷² is maximally phosphorylated using these conditions. The following antibodies were from Cell Signaling: rabbit anti-AMPKα1 (#2795), rabbit anti-AMPKα2 (#2757), mouse anti-AMPKα1/2 (#2793), rabbit anti-AMPKβ1/2 (#4150), rabbit anti-ACC (#3676), rabbit anti-pACC (#3661), rabbit anti-AMPKγ1 (#4187), rabbit anti-LKB1 (#3050) and rabbit anti-pThr¹⁷² (#2535). Mouse anti-vinculin was from Sigma–Aldrich (V9131). Mouse monoclonal anti-CaMKKβ antibody was a generous gift from Prof. Grahame Hardie (Dundee University).

Willows R., Sanders M.J., Xiao B., Patel B.R., Martin S.R., Read J., Wilson J.R., Hubbard J., Gamblin S.J, & Carling D. (2017). Phosphorylation of AMPK by upstream kinases is required for activity in mammalian cells. Biochemical Journal, 474(17), 3059-3073.

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Regulation of Survivin and Cell Cycle Proteins

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Sources were: YM155 (Selleck Chemicals); rabbit anti-Survivin IgG (#AF886) (R&D Systems); rabbit anti-P-Rb (S807/811, #9308), rabbit anti-P-Cyclin D1 (T286, #3300), mouse anti-Cyclin D1 (#2926), rabbit anti-Mcl-1(#5453), mouse anti-Cyclin D3 (#2936), rabbit anti-P-Akt (T308, #6955), rabbit anti-Akt (S473, #9271), rabbit anti-rS6 (#2217), rabbit anti-P-rS6 (S235/236, #2211), rabbit anti-P-p70S6K1 (T389, #9205), rabbit anti-mTOR (#2972), rabbit anti-P-mTOR (S2448, #2971), rabbit anti-Raptor (#4978, #4972) rabbit anti-P-Raptor (S792, #2083) rabbit anti-Rictor (#2140), rabbit anti-P-Rictor (T1135, #3806), rabbit anti-AMPKα (#5831), rabbit anti-P-AMPKα (T172, #2535) rabbit anti-AMPKα1 (#2795), rabbit anti-AMPKα2 (#2757), rabbit anti-4E-BP1 (#9644), rabbit anti-P-4E-BP1 (S65) (Cell Signaling Technologies); rabbit anti-Rb (sc-50), rabbit anti-Cyclin D1 (sc-753), rabbit anti-Cyclin D2 (sc-593), mouse anti-GAPDH (sc-51907), mouse anti-α-tubulin (sc-5386), mouse anti-P-rS6 (S240, sc-293143), mouse anti-rS6 (sc-74459), mouse anti-p70S6Kα (sc-39367) (Santa Cruz Biotechnologies, Inc.); mouse anti-β-actin (#A-5441), cycloheximide (CHX), actinomycin D (Act D), MG132, curcumin, (Sigma-Aldrich, Inc.); calyculin A, rapamycin and Compound C (LC labs, Inc.) DMEM/F12 (Media Tech); characterized fetal bovine serum (FBS) (HyClone).

Danielpour D., Gao Z., Zmina P.M., Shankar E., Shultes B.C., Jobava R., Welford S.M, & Hatzoglou M. (2019). Early Cellular Responses of Prostate Carcinoma Cells to Sepantronium Bromide (YM155) Involve Suppression of mTORC1 by AMPK. Scientific Reports, 9, 11541.

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