Pregm prostate epithelial cell growth medium

Manufactured by Lonza

PrEGM™ Prostate Epithelial Cell Growth Medium is a complete serum-free medium formulated for the culture and proliferation of human prostate epithelial cells. It contains essential nutrients, growth factors, and supplements required for optimal growth and maintenance of prostate epithelial cells in vitro.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) T medium, by Thermo Fisher Scientific (1 mentions) Hs27a, by American Type Culture Collection (1 mentions) Puromycin, by Thermo Fisher Scientific (1 mentions) Keratinocyte serum free medium, by Thermo Fisher Scientific (1 mentions) Lncap, by American Type Culture Collection (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Du145, by American Type Culture Collection (1 mentions)

2 protocols using pregm prostate epithelial cell growth medium

3D Co-culture of Prostate Cell Lines

Cited 3 times

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Human prostate cancer epithelial cell lines, LNCaP, C4-2, PC3, PC3M, and DU145, and a human bone stromal cell line HS27A (ATCC, Manassas, VA) that were used in our previous studies^{61 (link)–63 (link)} were maintained in T-medium (Invitrogen, Carlsbad, CA) with 5% fetal bovine serum (FBS; Invitrogen). Then, the 3D coculture of HS27A cells mixed with equal numbers of LNCaP or C4-2 cells or a monoculture was performed in an RCCS system (Synthecon, Houston, TX) following an established protocol^{21 (link)}. Each condition was performed in duplicate vessels for three independent experiments. Previously established CAFs and BAFs, derived from cancerous and benign/normal regions of the prostate gland, respectively^{21 (link)}, were confirmed as fibroblasts by positive vimentin but negative cytokeratin expression (Supplemental Fig. s6) and were maintained in PrEGM™ Prostate Epithelial Cell Growth Medium (Lonza, Walkersville, MD). All cells were cultured in a 37 °C incubator with 5% CO₂.

Hsieh C.L., Liu C.M., Chen H.A., Yang S.T., Shigemura K., Kitagawa K., Yamamichi F., Fujisawa M., Liu Y.R., Lee W.H., Chen K.C., Shen C.N., Lin C.C., Chung L.W, & Sung S.Y. (2017). Reactive oxygen species–mediated switching expression of MMP-3 in stromal fibroblasts and cancer cells during prostate cancer progression. Scientific Reports, 7, 9065.

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Prostate Cancer Cell Line Cultivation

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The human prostate cancer cell lines, DU145, PC3, LNCaP, and 293T, were from ATCC. Cells were cultured in RPMI1640 (Life Technologies) supplemented with 10% FBS (Life Technologies) and penicillin–streptomycin (100 U/ml) under 5% CO₂. Normal human prostate epithelial cells (PrEC, Lonza) were grown in PrEGM™ Prostate Epithelial Cell Growth Medium (Lonza), and human benign prostate cells RWPE-1 (henceforth referred as RWPE; ATCC) were grown in keratinocyte-serum free medium with supplements (Life Technologies) as specified by the supplier. Cells were established as free of mycoplasma and bacteria following instructions of ATCC. Lenti- and adenoviruses were generated by the University of Michigan Vector Core (Ann Arbor, MI). Prostate cancer cells were infected with lentiviruses expressing STMN1 or CtBP1 shRNAs or with nontargeting (NT)-shRNA controls; stable cell lines were generated by selection with 1 ug/mL puromycin (Thermo Fisher Scientific). For transient overexpression, PrEC cells were infected with adenoviruses expressing CtBP1 or lacZ.

Chakravarthi B.V., Chandrashekar D.S., Agarwal S., Balasubramanya S.A., Pathi S.S., Goswami M.T., Jing X., Wang R., Mehra R., Asangani I.A., Chinnaiyan A.M., Manne U., Sonpavde G., Netto G.J., Gordetsky J, & Varambally S. (2017). miR-34a Regulates Expression of the Stathmin-1 Oncoprotein and Prostate Cancer Progression. Molecular cancer research : MCR, 16(7), 1125-1137.

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