Y27632, PD98059, STO-609, and KN62 were purchased from Calbiochem (La Jolla, CA). 15% Tris-HCl Ready Gels and DC Protein Assay Kit were products of Bio-Rad (Hercules, CA), Myelin Basic Protein was purchased from Upstate Biotechnology (now Millipore, Billerica, MA) and [γ-32P]ATP from Perkin Elmer Life Sciences (Boston, MA). Antibody for MLCK and protein A/G agarose beads are products of Santa Cruz Biotechnology (Santa Cruz, CA). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).
Sprague-Dawley Rats were purchased from Charles River Laboratories and housed in the animal facility of the Division of Animal Resources, Virginia Commonwealth University. All procedures followed guidelines of and were in accordance with the Institutional Animal Care and Use Committee of Virginia Commonwealth University.
Sprague-Dawley Rats were purchased from Charles River Laboratories and housed in the animal facility of the Division of Animal Resources, Virginia Commonwealth University. All procedures followed guidelines of and were in accordance with the Institutional Animal Care and Use Committee of Virginia Commonwealth University.