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Mouse collagentype 3 col 3 elisa kit

Manufactured by R&D Systems

The Mouse Collagen Type III (Col III) ELISA Kit is a solid-phase enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse collagen type III in biological samples.

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2 protocols using mouse collagentype 3 col 3 elisa kit

1

Liver and Serum Collagen Quantification

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The levels of collagen I and III in the liver and serum samples were determined using commercially available ELISA kits (Mouse Collagentype I (Col I) ELISA kit and Mouse Collagentype III (Col III) ELISA kit); (R&D Systems, Minneapolis, MN). The serum levels of TIMP-1 and TGF-β1 were also determined using commercially available ELISA kits (R&D Systems, Minneapolis, MN). To prepare liver samples, up to100 mg of tissue was homogenized in 200μl of PBS and centrifuged at 2000–3000g for 20 min. The supernatant was collected and analyzed using an ELISA kit.
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2

Quantifying Liver and Serum Biomarkers

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The levels of TGF-β1, collagen I, and collagen III in the liver and serum samples were determined using commercially available ELISA kits including Mouse TGF-β1 Quantikine ELISA kit, Mouse Collagen type I (Col I) ELISA kit, and Mouse Collagen type III (Col III) ELISA kit (R&D Systems, Minneapolis, MN). The serum level of TGF-β1 was also determined using a commercially available ELISA kit (R&D Systems, Minneapolis, MN).
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