Organoid single cells were prepared 72 hr after nucleofection, lentivirus transduction or Lipofectamine transfection. Cells were analysed using Sony SH800S Cell Sorter and FlowJo software (version 10.4).
Sh800s cell sorter
Manufactured by FlowJo
The SH800S Cell Sorter is a flow cytometry-based instrument designed for cell sorting. It is capable of analyzing and separating cells based on their physical and fluorescent properties. The SH800S provides precise and reliable cell sorting performance to support a variety of applications in research and clinical settings.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sh800s cell sorter
1
Organoid Single-cell Analysis Protocol
2
Quantifying Cell Surface MMP14 Expression
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Following siRNA transfection, HAECs were trypsinized, washed, and incubated in the presence of MMP14 Alexa Fluor® 488-conjugated antibody (0.1 µg per 105 cells; #FAB9181G, R&D systems) or mouse IgG2B Alexa Fluor® 488-conjugated isotype control (0.1 µg per 105 cells; #IC0041G R&D systems) for 40 minutes in fluorescence-activated cell sorting (FACS) buffer containing 2% FBS. Cells were then washed 2Xs with ice-cold FACS buffer, suspended 1% paraformaldehyde and analyzed by flow cytometry on a SH800S Cell Sorter and FlowJo software. The median fluorescence intensity less the isotype control was compared for each data set for two independent experiments.
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