Cocktail inhibitor

Manufactured by Beyotime

Sourced in China

The Cocktail inhibitor is a laboratory reagent designed to inhibit a broad range of enzymes, including proteases, phosphatases, and other hydrolytic enzymes. It is formulated to provide a convenient and effective way to suppress unwanted enzyme activity in various experimental procedures.

Automatically generated - may contain errors

Lab products found in correlation

Pvdf membrane, by Merck Group (2 mentions) Bca method, by Thermo Fisher Scientific (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Odyssey clx equipment, by LI COR (1 mentions) Ripa buffer, by Solarbio (1 mentions)

Close spelling variants of this product

Phosphatase-protease inhibitor cocktails PMSF proteinase inhibitors cocktail Phosphatase inhibitors cocktail Cocktail of protease inhibitors Protease inhibitors cocktail Protease and phosphatase inhibitors cocktail Protease inhibitor cocktail for general use Proteinase and phosphatase inhibitor cocktail Protease/phosphatase inhibitor cocktail Proteinase inhibitor cocktail

2 protocols using cocktail inhibitor

Western Blot Analysis of Metabolic Regulators

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Tissue specimens or cell lines were used to extract total protein samples with RIPA lysis buffer with cocktail inhibitor (Beyotime, Nantong, China). After centrifuged, obtained protein samples were analyzed for their concentration using BCA method (Thermo Fisher Scientific). Sodium dodecyl sulfate–polyacrylamide gel electrophoresis was used to separate equal amount of protein per lane and separated proteins in the gels were transferred onto PVDF membranes (Millipore, Burlington, MA, USA). After 2 hours blocking with 5% non-fat milk at room temperature, the membranes were incubated overnight with primary antibodies against PDHA1, NRF1, peroxisome proliferator-activated receptor-γ coactivator 1-α (PGC1α), AP2α, and β-actin at 4 °C. Next, 2 h incubation with HRP-labeled secondary antibody was conducted at room temperature. All bands were detected with the chemiluminescence reagent (Pierce, Rockford, IL, USA) with β-actin as the internal reference.

Zhao L., Geng R., Huang Y., Zhang J., Cheng H., Zhou C, & Wang Y. (2023). AP2α negatively regulates PDHA1 in cervical cancer cells to promote aggressive features and aerobic glycolysis in vitro and in vivo. Journal of Gynecologic Oncology, 34(5), e59.

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Western Blot Analysis of Protein Lysates

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Tissues and cells were lysed in RIPA buffer (Solarbio, China) with a cocktail inhibitor (Beyotime, China). Protein lysates were subjected to SDS-PAGE and transfer onto PVDF membranes (Millipore, USA) was then carried out. Membrane blocking conducted, followed by incubation overnight using primary antibodies at 4 °C. Antibodies used in this study were shown in Additional file 3: Table S2. After being incubated using secondary antibodies, membranes were examined with Odyssey® CLx equipment (LI-COR, USA).

Ma G., Li G., Fan W., Xu Y., Song S., Guo K, & Liu Z. (2021). Circ-0005105 activates COL11A1 by targeting miR-20a-3p to promote pancreatic ductal adenocarcinoma progression. Cell Death & Disease, 12(7), 656.

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