All wild type isolates of E. amylovora and E. pyrifoliae used in this study are listed in Table 1 . All bacterial cultures were stored at −80 °C in Microbank cryobeads (Pro-Bank Diagnostics, Richmond Hill, ON, Canada). Initial subcultures were plated from cryobeads onto 2.3% Difco nutrient agar (NA; BD, Sparks, Maryland, USA), grown overnight at 27 °C and stored at 4 °C for up to a week. Working subcultures were plated on NA and grown either overnight at 27 °C or for 3 d at room temperature.
Difco nutrient agar na
Manufactured by BD
Sourced in United States
Difco nutrient agar (NA) is a general-purpose growth medium used for the cultivation of a wide range of microorganisms. It provides essential nutrients and a solidified matrix for the cultivation of bacteria, fungi, and other microbes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using difco nutrient agar na
1
E. amylovora and E. pyrifoliae Wild Type Isolates
2
Storage and Culturing of Bacterial Strains
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All bacterial strains used in this study are listed in Table 1 . All cultures were stored at −80 °C in Microbank cryobeads (Pro-Bank Diagnostics, Richmond Hill, ON, Canada). Cultures were initially spread onto 2.3% Difco nutrient agar (NA; BD, Sparks, MD, USA) using one cryobead. These cultures were grown overnight at 27 °C and stored at 4 °C for 1 to 2 weeks. Working subcultures were obtained from the initial cultures, plated onto NA, and grown at 27 °C overnight or over 2 to 3 days at room temperature.
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