Activity assay kits

Manufactured by Merck Group

Activity Assay Kits are a series of laboratory equipment designed to measure the activity of various enzymes, proteins, and other biological molecules. These kits provide a standardized and quantitative method to assess the functional properties of target analytes in a range of research and diagnostic applications.

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ALT and AST activity assay kits Enzyme activity assay kits ATPase Activity Assay Kit Colorimetric SOD activity assay kit Caspase-3 colorimetric activity assay kit Lipase Activity Assay Kit III Enzyme activity assay kit for β‐N‐acetyl‐glucosaminidase (NAG) Colorimetric activity assay kit Plasmin Activity Assay Kit (Fluorometric) Gpx Cellular activity assay Kit

2 protocols using activity assay kits

Liver Enzyme Levels in Diet Models

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The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were assessed using Activity Assay Kits (Sigma-Aldrich #MAK052 and MAK055 respectively) according to manufacturer's protocol. ALT and AST were detected in the serum of the HFD, LFD and HFD + TE mice collected from each time point. A T-test between the average of each group in every time point was used to determine the p-value.

Coia H., Ma N., Hou Y., Permaul E., Berry D.L., Cruz M.I., Pannkuk E., Girgis M., Zhu Z., Lee Y., Rodriquez O., Cheema A, & Chung F.L. (2020). Theaphenon E prevents fatty liver disease and increases CD4+ T cell survival in mice fed a high-fat diet. Clinical nutrition (Edinburgh, Scotland), 40(1), 110-119.

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Serum ALT and AST Assessment

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The levels of ALT and AST were assessed using Activity Assay Kits (Sigma-Aldrich #MAK052 and MAK055, respectively) according to manufacturer’s protocol. ALT and AST were detected in the serum of the HFD, LFD and HFD+TE mice collected and frozen at each time point.

Coia H., Ma N., Hou Y., Dyba M.D., Fu Y., Cruz M.I., Benitez C., Graham G.T., McCutcheon J.N., Zheng Y.L., Sun B., Kallakury B.V., Ma J., Fang H.B., Berry D.L., Muralidaran V, & Chung F.L. (2018). Prevention of lipid peroxidation-derived cyclic DNA adduct and mutation in high fat diet-induced hepatocarcinogenesis by Theaphenon E. Cancer prevention research (Philadelphia, Pa.), 11(10), 665-676.

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