Glacial acetic acid

Masson staining was employed to observe the collagen production and distribution at the contact surface between the screw and bone tissue. After sectioning as same as Hematoxylin-eosin staining section, the sections were dyed with hematoxylin dye, redyed with Masson Deli Chunhong acid reddish Staining Solution (Solarbio, China), immersed and differentiated in 2% glacial acetic acid (Servicebio, China) and 1% phosphomolybdic acid (Servicebio, China), then stained with aniline blue (Servicebio, China), leached by 2% glacial acetic acid, dehydrated by ethanol and hyalinized by xylene, finally sealed with neutral gum and observed under an inverted fluorescence microscope.

ROS, CAT, SOD (WST-8 method), the CCK-8 detection kit, and antioxidant NAC were purchased from Biyuntian Company (Shanghai, China). The POD detection kit, MC903, bovine serum albumin (BSA), cobalt acetylacetonate, dodecanol, octadecene, oleic acid, and oleylamine were purchased from Sigma (Shanghai, China). H₂O₂, ethanol, and isopropanol were purchased from Shenggong Company (Shenzhen, China). Rabbit anti-IgG and TSLP antibody (ab188766) were purchased from Abcam (Cambridge, UK). EDTA antigen retrieval solution, an immunohistochemistry kit, TB staining solution, and glacial acetic acid were purchased from Servicebio (Wuhan, China). Xylene and neutral gum were purchased from Sinopharm (Beijing, China). Dexamethasone (compound dexamethasone acetate gel) was purchased from Jinri Pharmaceutical Company (Xiamen, China).