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Ni nta agarose beads

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Ni-NTA agarose beads are a type of chromatography resin used for the purification of recombinant proteins. They consist of nickel-nitrilotriacetic acid (Ni-NTA) coupled to agarose beads, which allows for the selective binding of proteins with a histidine tag. The beads are designed to efficiently capture and purify these proteins from complex biological samples.

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Lab products found in correlation

Proteinase inhibitor cocktail, by Merck Group (3 mentions) Glutathione sepharose beads, by Macherey-Nagel (3 mentions) Anti histidine, by Santa Cruz Biotechnology (3 mentions) Anti actin, by Merck Group (3 mentions) Protein a g beads, by Merck Group (3 mentions) Anti gst, by Santa Cruz Biotechnology (3 mentions) Rnase a, by Merck Group (3 mentions) Anti ha, by Fortrea (3 mentions) Iscript cdna synthesis kit, by Bio-Rad (3 mentions) 35s methionine cysteine, by PerkinElmer (2 mentions) Mouse anti p53 1c12, by Cell Signaling Technology (2 mentions) Anti ppm1d, by Santa Cruz Biotechnology (2 mentions) Ppm1d inhibitor cct007093, by Merck Group (2 mentions) Adenosine 5 γ 32p triphosphate γ 32p atp, by PerkinElmer (2 mentions) Est clone clone id 5295521, by Thermo Fisher Scientific (2 mentions) Anti p53, by Santa Cruz Biotechnology (2 mentions) Anti rpl26, by Fortis Life Sciences (1 mentions) Anti tap73, by Fortis Life Sciences (1 mentions) α 32p utp, by PerkinElmer (1 mentions)

3 protocols using ni nta agarose beads

1

Characterization of PPM1D Protein Interactions

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Anti-p53, anti-PPM1D, anti-GST and anti-histidine were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-mouse p53 (1C12) was purchased from Cell Signaling Technology (Beverly, MA). Anti-HA was purchased from Covance (San Diego, CA). Anti-actin, proteinase inhibitor cocktail, RNase A, protein A/G beads, PPM1D inhibitor (CCT007093) were purchased from Sigma (St. Louis, MO). Anti-RBM38 antibody was made as previously described 1 (link). The Iscript cDNA synthesis kit was purchased from Bio-Rad Laboratories (Irvine, CA). 35S-methionine/cysteine and adenosine-5'-[γ-32P] Triphosphate ([γ-32P] ATP) were purchased from PerkinElmer (Waltham, MA). The Ni-NTA agarose beads were purchased from Biontex (Germany). The glutathione sepharose beads were purchased from Macherey-Nagel (Germany). The EST clone (clone ID 5295521), which contains a full length human PPM1D cDNA, was purchased from OpenBiosystems (Huntsville, AL).
Zhang M., Xu E., Zhang J, & Chen X. (2015). PPM1D phosphatase, a target of p53 and RBM38 RNA-binding protein, inhibits p53 mRNA translation via dephosphorylation of RBM38. Oncogene, 34(48), 5900-5911.
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2

Characterization of PPM1D Protein Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-p53, anti-PPM1D, anti-GST and anti-histidine were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-mouse p53 (1C12) was purchased from Cell Signaling Technology (Beverly, MA). Anti-HA was purchased from Covance (San Diego, CA). Anti-actin, proteinase inhibitor cocktail, RNase A, protein A/G beads, PPM1D inhibitor (CCT007093) were purchased from Sigma (St. Louis, MO). Anti-RBM38 antibody was made as previously described 1 (link). The Iscript cDNA synthesis kit was purchased from Bio-Rad Laboratories (Irvine, CA). 35S-methionine/cysteine and adenosine-5'-[γ-32P] Triphosphate ([γ-32P] ATP) were purchased from PerkinElmer (Waltham, MA). The Ni-NTA agarose beads were purchased from Biontex (Germany). The glutathione sepharose beads were purchased from Macherey-Nagel (Germany). The EST clone (clone ID 5295521), which contains a full length human PPM1D cDNA, was purchased from OpenBiosystems (Huntsville, AL).
Zhang M., Xu E., Zhang J, & Chen X. (2015). PPM1D phosphatase, a target of p53 and RBM38 RNA-binding protein, inhibits p53 mRNA translation via dephosphorylation of RBM38. Oncogene, 34(48), 5900-5911.
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3

Protein Purification and Analysis Protocol

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Anti-GST and anti-histidine were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-RPL26 and anti-TAp73 were purchased from Bethyl Laboratories (Montgomery, TX). Anti-HA was purchased from Covance (San Diego, CA). Anti-actin, proteinase inhibitor cocktail, RNase A, and protein A/G beads were purchased from Sigma (St. Louis, MO). The Iscript cDNA synthesis kit was purchased from Bio-Rad Laboratories (Irvine, CA). α-32P-UTP was purchased from PerkinElmer (Waltham, MA). The Ni-NTA agarose beads were purchased from Biontex (Germany). The glutathione sepharose beads were purchased from Macherey-Nagel (Germany).
Zhang M., Zhang J., Yan W, & Chen X. (2016). p73 expression is regulated by ribosomal protein RPL26 through mRNA translation and protein stability. Oncotarget, 7(48), 78255-78268.
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