For phenotypic analysis of dendritic cells: Fluorochrome conjugated antibodies CD14-PC5, CD80-FITC, HLADR-ECD, CD40-PE and CD86-PE conjugated were purchased from Beckman Coulter Inc (Carlsbad, CA, USA). The antibody against maturation marker CD83-conjugated to APC was purchased from Bio Legend (San Diego, CA, USA).
For phenotyping proliferating PBMCs, anti-CD56-PE, anti-CD4-PC5 anti-CD8-APC, anti- CD25-ECD and anti-CD3-PC7 antibodies were purchased from Beckman Coulter Inc (Carlsbad, CA, USA). Anti-FOXP3-PE was purchased from BioLegendInc (San Diego, CA, USA). Flow cytometry was performed as described previously [27 (link)]. All the samples were acquired using MoFlo XDP flow cytometer configured with three different lasers-blue (488 nm), violet (405 nm) red (640 nm) analyzed using Summit 5.2 software (both Beckman Coulter Inc, Carlsbad, CA, USA).
For phenotyping proliferating PBMCs, anti-CD56-PE, anti-CD4-PC5 anti-CD8-APC, anti- CD25-ECD and anti-CD3-PC7 antibodies were purchased from Beckman Coulter Inc (Carlsbad, CA, USA). Anti-FOXP3-PE was purchased from BioLegendInc (San Diego, CA, USA). Flow cytometry was performed as described previously [27 (link)]. All the samples were acquired using MoFlo XDP flow cytometer configured with three different lasers-blue (488 nm), violet (405 nm) red (640 nm) analyzed using Summit 5.2 software (both Beckman Coulter Inc, Carlsbad, CA, USA).