HEK 293T cells and U937 cells were cultured in DMEM and RPMI 1640 medium supplemented with 10% fetal bovine serum, respectively. Streptavidin-Agarose (S1638) and 5-Aza-CdR (A3656) were purchased from Sigma. Recombinant human TNFα (300-01A) was from Peprotech (Changchun, Jilin, China). Antibodies against p65 (sc-372) and p50 (sc-7178) were purchased from Santa Cruz Biotechnology (Changchun, Jilin, China). Goat Anti-Rabbit IgG (111-035-003) was from Jackson ImmunoResearch (Changchun, Jilin, China).
5 aza cdr a3656
Manufactured by Merck Group
5-Aza-CdR (A3656) is a chemical compound used in laboratory research. It functions as a cytidine analog, which can be used to study DNA methylation processes. The product is intended for research use only and its specific applications should be determined by the end user.
Automatically generated - may contain errors
2 protocols using 5 aza cdr a3656
1
NF-κB Pathway Activation Assay
2
Combinatorial Epigenetic and Cell Cycle Inhibition
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The DNA methyltransferase inhibitor 5-AZA-CdR (A3656) was purchased from Sigma Aldrich. Palbociclib isethionate (HY-A0065) was purchased from Med Chem Express. For in vitro experiments, cells were treated with 5-AZA-CdR (300 nM), palbociclib (250 nM) and vehicle (PBS or DMSO) as treatment. Cells were kept in culture for 5 and 7 days for treatment with 5-AZA-CdR and palbociclib respectively. For interferon blockade and exogenous interferon treatment experiments, cells were treated with either ruxolitinib (Invivogen tlrlrux, 1 mM) or IFN-b (R&D 8499-IF, 10 ng/mL) for 5 days. Cell viability was determined using a CellTiter-Glo luminescent cell viability assay (Promega). Data were presented as proliferation present by comparing the treated groups with the vehicle-treated cells.
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