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Mouse fgf 1

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Mouse FGF-1 is a recombinant protein that corresponds to the full-length mature form of basic fibroblast growth factor (FGF-1) from the mouse. FGF-1 is a heparin-binding growth factor that is involved in various cellular processes, such as angiogenesis, cell growth, and tissue repair.

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Lab products found in correlation

Cell culture products, by Thermo Fisher Scientific (2 mentions) Heparin, by Merck Group (2 mentions) Small molecule inhibitors, by Cayman Chemical (2 mentions) Human igf 2, by Merck Group (2 mentions) Mouse tpo, by Merck Group (2 mentions) Mouse scf, by Merck Group (2 mentions) Stemspan, by STEMCELL (2 mentions)

2 protocols using mouse fgf 1

1

Culture and Expansion of HSPCs

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293T cells were cultured in DMEM (high glucose) medium containing 10% FBS. K562 cells were cultured in IMDM medium plus 10% FBS. Enriched fresh hematopoietic stem and progenitor cells (HSPCs) were cultured in vitro in Stemspan (STEMCELL Technologies) supplemented with 10 μg/ml heparin (Sigma), 10 ng/ml mouse SCF, 20 ng/ml mouse TPO, 20 ng/ml human IGF-II, 10 ng/ml mouse FGF-1, and 100 ng/ml human Angptl3 25 . All recombinant proteins were purchased from PEPROTECH or PROSPEC companies. All cell culture products were purchased from Life Technologies unless otherwise mentioned. All small molecule inhibitors were purchased from Cayman Chemicals.
Zhang Z., Zhu P., Zhou Y., Sheng Y., Hong Y., Xiang D., Qian Z., Mosenson J, & Wu W.S. (2016). A Novel Slug-containing Negative Feedback Loop regulates SCF/c-Kit-Mediated Hematopoietic Stem Cell Self-renewal. Leukemia, 31(2), 403-413.
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2

Culture and Expansion of HSPCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
293T cells were cultured in DMEM (high glucose) medium containing 10% FBS. K562 cells were cultured in IMDM medium plus 10% FBS. Enriched fresh hematopoietic stem and progenitor cells (HSPCs) were cultured in vitro in Stemspan (STEMCELL Technologies) supplemented with 10 μg/ml heparin (Sigma), 10 ng/ml mouse SCF, 20 ng/ml mouse TPO, 20 ng/ml human IGF-II, 10 ng/ml mouse FGF-1, and 100 ng/ml human Angptl3 25 . All recombinant proteins were purchased from PEPROTECH or PROSPEC companies. All cell culture products were purchased from Life Technologies unless otherwise mentioned. All small molecule inhibitors were purchased from Cayman Chemicals.
Zhang Z., Zhu P., Zhou Y., Sheng Y., Hong Y., Xiang D., Qian Z., Mosenson J, & Wu W.S. (2016). A Novel Slug-containing Negative Feedback Loop regulates SCF/c-Kit-Mediated Hematopoietic Stem Cell Self-renewal. Leukemia, 31(2), 403-413.
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