Licor odyssey m

Manufactured by LI COR

The LiCOR Odyssey M is a compact, high-resolution fluorescence imager designed for a variety of applications in life science research. It is capable of detecting and quantifying fluorescent signals from various sample types, including gels, blots, and microplates.

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Lab products found in correlation

Gradient gel, by Bio-Rad (1 mentions) Bio safe coomassie g 250, by Bio-Rad (1 mentions) Biorad precision plus protein kaleidoscope prestained protein standards, by Bio-Rad (1 mentions) Empiria software, by LI COR (1 mentions) Intercept blocking buffer, by LI COR (1 mentions) Mouse anti flag m2, by Merck Group (1 mentions) Anti mouse irdye 680rd, by LI COR (1 mentions) Anti rabbit irdye 800cw, by LI COR (1 mentions) Rabbit anti actin, by Fortis Life Sciences (1 mentions)

2 protocols using licor odyssey m

Visualization of Amyloid-β Oligomers

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Soluble amyloid-β oligomer preparations were solubilized in Laemmli Sample buffer (1X final concentration), boiled for 5 minutes at 96°C, and analyzed by SDS-PAGE. The prepared samples (20 μL) were loaded into a 12-well 4–20% gradient gel (BioRad Laboratories, Hercules CA, Cat No. 4561095) along with BioRad Precision Plus Protein Kaleidoscope Prestained Protein Standards (BioRad Laboratories, Hercules CA, Cat No. 1610375) and electrophoresed at 170V for 50 minutes. The gel was washed three times for 5 minutes each with deionized water, stained with BioSafe Coomassie G-250 (BioRad Laboratories, Hercules CA, Cat No. 1610786) with gentle shaking for 1h, destained overnight, and imaged on a LiCOR Odyssey M (LiCOR Biosciences, Lincoln NE). Images were collected and extracted using Empiria software (LiCOR Biosciences, Lincoln NE).

Haynes J.R., Whitmore C.A., Behof W.J., Landman C.A., Ong H.H., Feld A.P., Suero I.C., Greer C.B., Gore J.C., Wijesinghe P., Matsubara J.A., Wadzinski B.E., Spiller B.W, & Pham W. (2024). TARGETING SOLUBLE AMYLOID-BETA OLIGOMERS WITH A NOVEL NANOBODY. Research Square.

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Western Blot Analysis of Cellular Proteins

Cited 1 time

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Protein was collected from cells as previously described (28 (link)). Membranes were blocked in intercept blocking buffer (Li-COR Biosciences). Immunoblotting was performed using mouse anti-FLAG M2 (Sigma-Aldrich), rabbit anti-Actin (Bethyl), or mouse anti-DCAF1 (Proteintech) for 1hr. Blots were incubated with secondary antibodies IRDye 800CW anti-Rabbit and IRDye 680RD anti-Mouse (Li-COR Biosciences) for 1 hr and then visualized using the Li-COR Odyssey M (Li-COR Biosciences).

Sandoval C, & Fregoso O.I. (2023). HIV-1 Vpr-induced DNA damage activates NF-κB independent of cell cycle arrest and CRL4ADCAF1 engagement. bioRxiv.

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