The 19-nortestosterone, estradiol (E2), trenbolone (TRE), methyltestosterone (MT) and testosterone propionate (TP) were purchased from Dr. Ehrenstorfer GmbH (Augsburg, Germany). testosterone (TES) and nandrolone phenylpropionate (NPP) were purchased from MACKLIN (Shanghai, China). The keyhole limpet hemocyanin (KLH), bovine serum albumin (BSA), ovalbumin (OVA), Freund’s complete adjuvants and Freund’s incomplete adjuvants were purchased from Sigma (St. Louis, MO, USA). The conjugates 19-NT-KLH and 19-NT-OVA were prepared in our laboratory (Figure 1 ). The TRIzol reagent was from Thermo Fisher Scientific (Shanghai, China). The first strand cDNA synthesis kit was purchased from TaKaRa (Dalian, China). The gel extraction and PCR purification kit were obtained from QIAGEN (Dusseldorf, Germany). The molecular biology reagents, including helper phage M13K07, restriction enzymes, T4 DNA ligase and others, were purchased from New England Biolabs (Ipswich, MA, USA). The rabbit anti-camelid VHH antibody-HRP (anti-VHH-HRP) was purchased from GenScript (Nanjing, China).
19 nortestosterone
Manufactured by LGC
Sourced in Germany
19-nortestosterone is an endogenous anabolic-androgenic steroid. It is the 19-nor derivative of the natural male hormone testosterone. The core function of 19-nortestosterone is to serve as a precursor for the synthesis of other steroid hormones.
Automatically generated - may contain errors
Lab products found in correlation
First strand cdna synthesis kit, by Takara Bio (1 mentions)
T4 dna ligase, by New England Biolabs (1 mentions)
M13k07 helper phage, by New England Biolabs (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Restriction enzyme, by New England Biolabs (1 mentions)
Rabbit anti camelid vhh antibody hrp anti vhh hrp, by GenScript (1 mentions)
Incomplete freund s adjuvant, by Merck Group (1 mentions)
Sodium chloride (nacl), by Beijing Chemical Works (1 mentions)
17β estradiol, by LGC (1 mentions)
Chloroauric acid haucl4, by Merck Group (1 mentions)
Methanol, by Beijing Chemical Works (1 mentions)
Estrone, by Merck Group (1 mentions)
Estriol, by Merck Group (1 mentions)
Bisphenol a, by LGC (1 mentions)
Sodium citrate c6h5na3o7, by Merck Group (1 mentions)
2 protocols using 19 nortestosterone
1
Synthesis and Validation of Camelid VHH Antibodies
2
Synthesis and Characterization of Estradiol Aptamer
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The sequences of the 17β-estradiol binding aptamer26 (link) and the split P1 and P2 fragments were synthesized by Shanghai Sangon Biotechnology Co., Ltd. (Shanghai, China), as listed in Table 1 . The lyophilized powder was dissolved in ultrapure water and stored at 4°C before use. The concentration of the oligonucleotide was determined by measuring the UV absorbance at 260 nm. Estriol, estrone, chloroauric acid (HAuCl4), and sodium citrate (C6H5Na3O7) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Bisphenol A, 17β-estradiol, 19-nortestosterone, and diethylstilbestrol were purchased from Dr. Ehrenstorfer GmbH (Augsburg, Germany). Other chemicals including sodium chloride and methanol were all purchased from Beijing Chemical Reagent Company (Beijing, China). All of the chemicals were of at least analytical grade.
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