For purposes of lipolysis assessment, glycerol concentration in dialysate was determined and used as a marker of lipolysis (Free glycerol reagent F6428, Sigma-Aldrich, St. Louis, MI, USA). FFA in serum were measured by NEFA-HR2 assay (Wako Chemical Inc., Richmond, VA, USA). All the other biochemical analyses were performed by the institutional Department of Laboratory Diagnostics, Kralovske Vinohrady University Hospital, Prague.
Free glycerol reagent f6428
Manufactured by Merck Group
Sourced in United States
Free glycerol reagent F6428 is a laboratory product manufactured by Merck Group. It is used to determine the concentration of free glycerol in a sample. The reagent works by enzymatically converting glycerol to dihydroxyacetone phosphate and hydrogen peroxide, which is then measured colorimetrically.
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Lab products found in correlation
2 protocols using free glycerol reagent f6428
1
Lipolysis Assessment Protocol
2
Liver Lipid Quantification by Oil Red O
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Liver frozen sections were prepared using a Leica cryostat (Leica Biosystems Nussloch GmbH, Nussloch, Germany) and stained with 1% Oil red O. Liver samples were homogenized in 1% acetic acid and extracted by chloroform:methanol (2:1). The organic phase was dried by evaporation and dissolved in isopropanol. TAG was completely hydrolyzed with ethanolic KOH, and glycerol was measured using a free glycerol reagent (Sigma, Free Glycerol Reagent F‐6428). TAG levels were calculated and normalized to liver weight.
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