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Protein estimation reagent

Manufactured by Bio-Rad
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The Protein estimation reagent is a colorimetric assay used to determine the concentration of protein in a sample. It measures the total protein content based on the reaction between the protein and the reagent, resulting in a color change that can be quantified spectrophotometrically.

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Lab products found in correlation

Page reagents, by Bio-Rad (4 mentions) Rhodamine phalloidin, by Merck Group (4 mentions) Cy2 and cy3 conjugated secondary antibodies, by Jackson ImmunoResearch (3 mentions) Molecular weight standards for proteins, by Bio-Rad (3 mentions) Antibody to lpl sc 16657 goat, by Santa Cruz Biotechnology (2 mentions) Antibodies to gapdh, by R&D Systems (2 mentions) Hrp conjugated secondary antibodies for immunoblotting, by GE Healthcare (2 mentions) Molecular weight standard, by Bio-Rad (1 mentions) Tnfr1, by R&D Systems (1 mentions) Alizarin red solution, by Lifeline Cell Technology (1 mentions) M csf 416 ml, by R&D Systems (1 mentions) Molecular weight protein standards, by Bio-Rad (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Nucleoporin c39a3, by Cell Signaling Technology (1 mentions) Cd44 icd antibody kal ko601, by Cosmo Bio (1 mentions) Alexa fluor 488 4412, by Cell Signaling Technology (1 mentions) Prolong gold antifade dapi 8961, by Cell Signaling Technology (1 mentions)

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Protein reagents

5 protocols using protein estimation reagent

1

Immunoblotting and Immunofluorescence Reagents

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Antibody to LPL (SC-16657; Goat) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies to GAPDH and TNF-α receptor 1 (TNFR1) were purchased from R & D Systems (Minneapolis, MN). Protein estimation reagent, molecular weight standards, and PAGE reagents were bought from Bio-Rad (Hercules, CA). Cy2- and Cy3-conjugated secondary antibodies were purchased from Jackson Immunoresearch (West Grove, PA). HRP-conjugated secondary antibodies for immunoblotting and the phosphoserine (p-Serine) antibody were purchased from Abcam (Cambridge, MA). Mounting solutions for mounting of coverslips were purchased from Thomas Scientific (Swedesboro, NJ) or Vector Labs (Burlingame, CA). Rhodamine-phalloidin and all other chemicals were purchased from Sigma (St. Louis, MO).
Chellaiah M.A., Moorer M.C., Majumdar S., Aljohani H., Morley S.C., Yingling V, & Stains J.P. (2020). L-Plastin deficiency produces increased trabecular bone due to attenuation of sealing ring formation and osteoclast dysfunction. Bone Research, 8, 3.
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2

Antibody Acquisition and Characterization

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Antibody to L-plastin (SC-16657; Goat) was bought from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibodies to GAPDH was purchased from R & D Systems (Minneapolis, MN) and Sigma (St. Louis, MO). Protein estimation reagent, molecular weight standards for proteins, and PAGE reagents were bought from Bio-Rad. Cy2- and Cy3-conjugated secondary antibodies were purchased from Jackson Immunoresearch (West Grove, PA). HRP-conjugated secondary antibodies for immunoblotting were obtained from GE Healthcare. Antibody to phosphoserine (p-Serine) was bought from Zymed laboratories (61–8100) or Millipore (AB1603). Alizarin red solution was bought from Life-line Cell Technology (CM-0058; Fredrick, MD) Rhodamine-phalloidin and other chemicals were purchased from Sigma (St. Louis, MO).
Chellaiah M.A., Majumdar S, & Aljohani H. (2018). Peptidomimetic inhibitors of L-plastin reduce the resorptive activity of osteoclast but not the bone forming activity of osteoblasts in vitro. PLoS ONE, 13(9), e0204209.
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3

Formulation and Characterization of PLGA-PEG Nanoparticles

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Methoxy terminated PLGA-PEG (10:5 kDa) was purchased from Polyscitech (West Lafayette, IN). Polyvinyl alcohol (PVA, 25 kDa) was purchased from Polysciences (Warrington, PA). Antibody to L-plastin (SC-16657) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Phosphoserine (125277) antibody was bought from Abcam (Cambridge, MA). Recombinant TNF-α (216-TA) and MCSF (416-ML) were purchased from R&D Systems (Minneapolis, MN). Protein estimation reagent, molecular weight standards for proteins, and PAGE reagents were bought from Bio-Rad. HRP-conjugated secondary antibodies for immunoblotting were obtained from GE Healthcare. PLGA (7-17 kDa, 50:50), dichloromethane (DCM), anti-rabbit antibodies to GAPDH, Rhodamine-phalloidin, and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO). sNT-LPL peptides (fluorescence-conjugated and unconjugated) are made from Genscript Co. (Piscataway (NJ)).
Majumdar S., Wadajkar A.S., Aljohani H., Reynolds M.A., Kim A.J, & Chellaiah M. (2019). Engineering of L-Plastin Peptide-Loaded Biodegradable Nanoparticles for Sustained Delivery and Suppression of Osteoclast Function In Vitro. International Journal of Cell Biology, 2019, 6943986.
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4

Multiplex Immunodetection of Key Proteins

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Antibodies to CD44 (156-3C11), RUNX2 (D1L7F), SOX2 (D6D9), Ezrin (3145S) MMP-9 (D6O3H) and Nucleoporin (C39A3) were purchased from Cell Signaling Technology, Inc. (Danvers, MA). Androgen Receptor (sc-7305) and RUNX2 (sc-390351) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX). CD44-ICD antibody (KAL-KO601) was purchased from Cosmo Bio (Tokyo, Japan). Antibody to GAPDH (G9545) and other chemicals were purchased from Sigma-Aldrich, Inc. (St.Louis, MO). HRP-conjugated Anti-Rabbit and anti-Mouse secondary antibodies were obtained from Kirkegaard & Perry Laboratories (Gaithersburg, MD) and Santa Cruz Biotechnology (Dallas, TX), respectively. Protein estimation reagent, molecular weight protein standards, and reagents for polyacrylamide gel electrophoresis were purchased from Bio-Rad (Hercules, CA). Polyvinylidene difluoride membrane was obtained from Millipore Corp. (Bedford, MA). ECL reagent was purchased from Pierce (Rockford, IL). The fluorochrome-conjugated secondary antibody Alexa Fluor 488 (4412) and ProLong Gold Antifade DAPI (8961) were obtained from Cell Signaling Technology, Inc. (Danvers, MA).
Senbanjo L.T., AlJohani H., Majumdar S, & Chellaiah M.A. (2019). Characterization of CD44 intracellular domain interaction with RUNX2 in PC3 human prostate cancer cells. Cell Communication and Signaling : CCS, 17, 80.
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5

Comprehensive Antibody Source Protocol

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Antibody to LPL (SC-16657; Goat) was bought from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibodies to GAPDH and TNF-α receptor 1 (TNFR1) were purchased from R & D Systems (Minneapolis, MN). Protein estimation reagent, molecular weight standards for proteins, and PAGE reagents were bought from Bio-Rad (Hercules, CA). Molecular weight markers have also been bought from Promega (Madison, WI). Cy2- and Cy3-conjugated secondary antibodies were purchased from Jackson Immunoresearch (West Grove, PA). HRPconjugated secondary antibodies for immunoblotting and phosphoserine (p-Serine) antibody were bought from Abcam (Cambridge, MA). Mounting solutions for mounting of coverslips were bought from Thomas Scientific (Swedesboro, NJ) or Vector Labs (Burlingame, CA). Rhodaminephalloidin and all other chemicals were purchased from Sigma (St. Louis, MO).
Chellaiah M.A., Ma T, & Majumdar S. (2018). L-Plastin Phosphorylation Regulates the Early Phase of Sealing Ring Formation by Actin Bundling Process in Mouse Osteoclasts. Experimental cell research, 372(1), 73-82.
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