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Single cell immunology v2 kit

Manufactured by NanoString
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The Single-cell immunology v2 kit is a laboratory equipment product designed for single-cell analysis. It provides tools and reagents to enable the study of immune cells at the individual cell level.

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Lab products found in correlation

Ncounter max, by NanoString (2 mentions) Rlt buffer, by Qiagen (2 mentions)

2 protocols using single cell immunology v2 kit

1

Comprehensive Neutrophil Transcriptome Analysis

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Purified blood neutrophils were stored in RLT buffer (Qiagen) with 1% 2-mercaptoethanol (Sigma Aldrich) at −80 °C until RNA extraction as above. The single-cell immunology v2 kit (NanoString) was used with 20 pre-amp cycles for all samples. Hybridized samples were prepared on a Prep Station and scanned on a nCounter MAX (NanoString). Raw counts were analyzed using DESeq2 with internal normalization, which gave lower variance than normalizing to included housekeeping genes. DEGs were identified using a model matrix correcting for repeated individual measurements.
Jochems S.P., Marcon F., Carniel B.F., Holloway M., Mitsi E., Smith E., Gritzfeld J.F., Solórzano C., Reiné J., Pojar S., Nikolaou E., German E.L., Hyder-Wright A., Hill H., Hales C., de Steenhuijsen Piters W.A., Bogaert D., Adler H., Zaidi S., Connor V., Gordon S.B., Rylance J., Nakaya H.I, & Ferreira D.M. (2018). Inflammation induced by influenza virus impairs human innate immune control of pneumococcus. Nature Immunology, 19(12), 1299-1308.
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2

Profiling Blood Neutrophil Transcriptomes

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Purified blood neutrophils were stored in RLT buffer (Qiagen) with 1% 2-mercaptoethanol (Sigma) at -80C until RNA extraction as above. The single cell immunology v2 kit (Nanostring) was used with 20 pre-amp cycles for all samples. Hybridized samples were prepared on a Prep Station and scanned on a nCounter® MAX (Nanostring). Raw counts were analysed using DESeq2 using internal normalization, which gave lower variance than normalizing to included housekeeping genes. DEG were identified using a model matrix correcting for repeated individual measurements.
Jochems S.P., Marcon F., Carniel B.F., Holloway M., Mitsi E., Smith E., Gritzfeld J.F., Solórzano C., Reiné J., Pojar S., Nikolaou E., German E.L., Hyder-Wright A., Hill H., Hales C., de Steenhuijsen Piters W.A., Bogaert D., Adler H., Zaidi S., Connor V., Gordon S.B., Rylance J., Nakaya H.I, & Ferreira D.M. (2018). Inflammation induced by influenza virus impairs human innate immune control of pneumococcus. Nature Immunology, 19(12), 1299-1308.
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