1260 infinity high performance liquid chromatography system

Cellular fatty acids were analysed using cell material after incubation on TSA for 48 h at 30 °C with the method described by Sasser [9] . Fatty acid methyl ester extracts were analysed by gas chromatography-mass spectrometry as described by Wiertz et al. [3] . Polar lipids and isoprenoid quinones were analysed as described by Minnikin et al. [10] from cells incubated in TSB at 30 °C for 24 h. Cells were harvested at an OD 600 of 0.8-1.2 by centrifugation (17000 g, 4 °C, 10 min, Sorvall LYNX 4000 Centrifuge, Thermo Scientific), and washed twice in Ringer's solution. Polar lipids were separated and detected by 2D thin-layer chromatography as described before [11] . Molybdenum blue, ninhydrin and α-naphthol were used to visualize phospholipids, lipids with free amino groups, and glycolipids, respectively, while primuline was used to visualize all polar lipids under UV light. Cell pellets were air dried, and isoprenoid quinones were extracted from about 10 mg dry mass. Menaquinones were detected using an 1260 Infinity high performance liquid chromatography System (Agilent Technologies) equipped with an ODS Hypersil (5 µm, 250×4mm) column and a diode array detector, using the parameters described by Wiertz et al. [3] . Vitamin K 1 was used as an external standard. Mycolic acids were extracted from lyophilized biomass as described by Wiertz et al. [3] .