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Cd3 e660

Manufactured by Thermo Fisher Scientific
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The CD3-e660 is a laboratory instrument designed for detecting and analyzing CD3 proteins in biological samples. It utilizes flow cytometry technology to provide quantitative measurements of CD3 expression levels.

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Lab products found in correlation

B220 apc e780, by Thermo Fisher Scientific (2 mentions) Mac 1 pe cy7, by Thermo Fisher Scientific (2 mentions) Cd45.2 fitc, by Thermo Fisher Scientific (2 mentions) Ter119 pe cy5, by Thermo Fisher Scientific (2 mentions) Gr 1 pb, by Thermo Fisher Scientific (2 mentions)

2 protocols using cd3 e660

1

Fetal Hematopoietic Stem Cell Transplantation

Cited 1 time
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Fetal HSCs (Lin-/Sca-1+/c-Kit+/Flk2-/CD150+/CD48-) were isolated from the fetal livers of E14.5 Mcm3+/+, Mcm3+/Lox or Mcm3Lox/Lox CD45.2 C57Bl/6 donor mice, and transplanted into lethally irradiated CD45.1 C57Bl/6 primary recipients (50 fetal HSCs per mouse; 5 mice per group) together with 300,000 Sca-1-depleted CD45.1 helper BM cells. 4 months post-transplantation, the primary recipients were sacrificed to assess the percentage of donor-derived chimerism in the bone marrow (BM), spleen, peripheral blood and HSC compartment and to re-isolate donor-derived adult MCM3+/+ or MCM3Lox/Lox HSCs53 (link). Donor chimerism was analyzed using CD45.2-FITC, (eBioscience 12-0453-83; 1:50), B220-APC-e780 (47-0452-82; 1:800), Gr-1-PB (1:400), Mac-1-PE-Cy7 (1:3200), CD3-e660 (eBioscience, 50-0032-82; 1:400) and Ter-119-PE-Cy5 (eBioscience 15-5921-83; 1:800) antibodies. Re-isolated CD45.2+ donor-derived adult MCM3+/+ or MCM3Lox/Lox HSCs were transplanted into lethally irradiated CD45.1 secondary recipients (500 HSCs per mouse; 5 mice per group) together with 300,000 Sca-1-depleted CD45.1 helper BM cells.
Alvarez S., Díaz M., Flach J., Rodriguez-Acebes S., López-Contreras A.J., Martínez D., Cañamero M., Fernández-Capetillo O., Isern J., Passegué E, & Méndez J. (2015). Replication stress caused by low MCM expression limits fetal erythropoiesis and hematopoietic stem cell functionality. Nature Communications, 6, 8548.
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2

Characterizing Fetal HSC Function via Transplantation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Fetal HSCs (Lin−/Sca-1+/c-Kit+/Flk2−/CD150+/CD48−) were isolated from the fetal livers of E14.5 Mcm3+/+, Mcm3+/Lox or Mcm3Lox/Lox CD45.2 C57Bl/6 donor mice, and transplanted into lethally irradiated CD45.1 C57Bl/6 primary recipients (50 fetal HSCs per mouse; 5 mice per group) together with 300,000 Sca-1-depleted CD45.1 helper BM cells. 4 months post-transplantation, the primary recipients were sacrificed to assess the percentage of donor-derived chimerism in the bone marrow (BM), spleen, peripheral blood and HSC compartment and to re-isolate donor-derived adult MCM3+/+ or MCM3Lox/Lox HSCs53 (link). Donor chimerism was analyzed using CD45.2-FITC, (eBioscience 12-0453-83; 1:50), B220-APC-e780 (47-0452-82; 1:800), Gr-1-PB (1:400), Mac-1-PE-Cy7 (1:3200), CD3-e660 (eBioscience, 50-0032-82; 1:400) and Ter-119-PE-Cy5 (eBioscience 15-5921-83; 1:800) antibodies. Re-isolated CD45.2+ donor-derived adult MCM3+/+ or MCM3Lox/Lox HSCs were transplanted into lethally irradiated CD45.1 secondary recipients (500 HSCs per mouse; 5 mice per group) together with 300,000 Sca-1-depleted CD45.1 helper BM cells.
Alvarez S., Díaz M., Flach J., Rodriguez-Acebes S., López-Contreras A.J., Martínez D., Cañamero M., Fernández-Capetillo O., Isern J., Passegué E, & Méndez J. (2015). Replication stress caused by low MCM expression limits fetal erythropoiesis and hematopoietic stem cell functionality. Nature Communications, 6, 8548.
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