H9c2 cells were homogenized in RIPA buffer (PBS, 1% Ipegal, 0.5% sodium deoxycholate, and 0.1% SDS) with proteinase and phosphatase inhibitor cocktails (Sigma-Aldrich, St. Louis, MO). Protein concentration was determined by the Bradford assay (Pierce, Rockford, IL) and stored at −70°C until use. Primary antibodies used for Western blot included CD36 [3313; Cell Signaling Technologies (CSTs), Danvers, MA], Leptin Ab, and Na-ATPase, cell membrane-specific. Donkey anti-rabbit (711-035-152; Jackson ImmunoResearch Laboratories, West Grove, PA) was used as the secondary antibody.
Donkey anti rabbit 711 035 152
Manufactured by Jackson ImmunoResearch
Sourced in Cameroon, United States
Donkey anti-rabbit (711-035-152) is a secondary antibody produced in donkeys and specifically reactive to rabbit immunoglobulins. It is intended for use in immunological techniques such as Western blotting, ELISA, and immunohistochemistry where detection of rabbit primary antibodies is required.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using donkey anti rabbit 711 035 152
1
Protein Extraction and Western Blot
2
Lung Tissue Protein Extraction and Western Blot
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Mouse lung tissue was homogenized in RIPA buffer (PBS, 1% Ipegal, 0.5% sodium deoxycholate, 0.1% SDS) with proteinase and phosphatase inhibitor cocktails (Sigma-Aldrich). Protein concentration was determined by Bradford assay (Pierce, CO, USA) and was stored at −70 °C until use. Primary antibodies used for Western blot included MSX1 (ab49153, Abcam) and HSC70/HSP73, referred to as HSP70 (ADI-SPA-816, Enzo). Donkey anti-rabbit (711-035-152, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) was used as secondary antibody.
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