Mouse anti egfp

Manufactured by Roche

The Mouse anti-eGFP is a monoclonal antibody that specifically binds to the enhanced green fluorescent protein (eGFP). This antibody can be used for the detection and localization of eGFP-tagged proteins in various applications.

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Lab products found in correlation

A11001, by Thermo Fisher Scientific (1 mentions) A11010, by Thermo Fisher Scientific (1 mentions) Rabbit anti dsred, by Takara Bio (1 mentions) B27 supplement, by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Rabbit anti vgat, by Synaptic Systems (1 mentions) Mouse anti synapsin, by Synaptic Systems (1 mentions)

Close spelling variants of this product

Anti-EGFP (4 citations) Anti-EGFP mouse monoclonal antibody (2 citations)

2 protocols using mouse anti egfp

Whole-mount Immunostaining of Embryos

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Embryos were fixed for 1-2 h at room temperature in 4% PFA, washed for 3×5 min with 1×PBS, 0.2% Triton X-100, head and tail were cut off and the gut was cut open. Embryos were incubated in blocking buffer containing 1% DMSO, 1% sheep serum, 1% BSA and 1% Triton X-100 in 1×PBS for at least 60 min at room temperature. The embryos were then incubated overnight at 4°C with primary and secondary antibodies, using 1:200 and 1:1000 dilutions, respectively. Primary antibodies: rabbit anti-carboxypeptidase A/CPA (Chemicon, AB1213), mouse anti-eGFP (Roche, 11814460001), rabbit anti-dsRed (Clontech, 632496). Alexa Fluor 488 and Alexa Fluor 546 conjugated anti-rabbit and anti-mouse were used as secondary antibodies (Invitrogen, A11001, A11010).

Schmitner N., Kohno K, & Meyer D. (2017). ptf1a+, ela3l− cells are developmentally maintained progenitors for exocrine regeneration following extreme loss of acinar cells in zebrafish larvae. Disease Models & Mechanisms, 10(3), 307-321.

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Striatal Mass Cultures Western Blotting

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Western blotting was done on striatal mass cultures which were prepared according to the same protocols as autaptic cultures, 2,25 but plated on poly-L-lysine at a density of 7000 cells/cm 2 and kept in Dulbecco's modified eagle's medium with GlutaMAX TM (GIBCO, 31966-021) with 10% fetal bovine serum and B27-supplement (Gibco, 17504-044) for DIV 1.
Cultures were infected on DIV 2 and harvested on DIV 9. Antibodies used for Western blotting were mouse-anti-Synapsin (Synaptic Systems, 106011) at 1:6000, rabbit-anti-VGAT (Synaptic Systems, 131013) at 1:1000 and mouse-anti-EGFP (Roche, 11814460001) at 1:3000.

Platzer K., Sticht H., Bupp C., Ganapathi M., Pereira E.M., Guyader G.L., Bilan F., Henderson L.B., Taschenberger H., Brose N., Jamra R.A., & Wojcik S.M. (2021). De novo missense variants in SLC32A1 cause a neurodevelopmental disorder with epilepsy due to impaired GABAergic neurotransmission.

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