Whole cell lysate was prepared in RIPA lysis buffer with 1X Laemmli buffer, then denatured by heating at 100°C for 5 minutes. Denatured proteins were separated in SDS-PAGE gel and transferred onto PVDF membrane. The PVDF membrane was incubated in TBST with 5% nonfat dry milk for one hour at room temperature prior to incubating with primary antibody of interest overnight at 4°C with gentle rotation. Appropriate horse radish peroxidase conjugated secondary antibody was applied for one hour at room temperature. Signal was developed by applying SuperSignal West Pico chemiluminescence substrate and captured using the ChemiDoc imaging instrument. Primary antibodies used in this study: anti-AHR antibody (BML-SA210 – Enzo Biochem, Farmingdale, NY), anti-p27Kip1 antibody (610242 – BD Biosciences), anti-p53 antibody (DO-1 sc-126 – Santa Cruz Biotechnology, Dallas, TX), anti-p21 antibody (2947S – Cell Signaling Technology, Danvers, MA), and anti-GAPDH antibody (sc-36502, Santa Cruz).
Anti ahr antibody bml sa210
Manufactured by Enzo Life Sciences
The Anti-AHR antibody (BML-SA210) is a laboratory research tool produced by Enzo Life Sciences. It is designed to recognize and bind to the aryl hydrocarbon receptor (AhR), a transcription factor that plays a role in the regulation of gene expression in response to environmental stimuli. The antibody can be used for various research applications that involve the detection and study of the AhR protein.
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2 protocols using anti ahr antibody bml sa210
1
Western Blot Analysis of Cell Signaling Proteins
2
Western Blot Analysis of Cellular Proteins
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Whole cell lysate was prepared in RIPA lysis buffer with 1X Laemmli buffer, then denatured by heating at 100°C for 5 minutes. Denatured proteins were separated in SDS-PAGE gel and transferred onto PVDF membrane. The PVDF membrane was incubated in TBST with 5% nonfat dry milk for one hour at room temperature prior to incubating with primary antibody of interest overnight at 4°C with gentle rotation. Appropriate horse radish peroxidase conjugated secondary antibody was applied for one hour at room temperature. Signal was developed by applying SuperSignal West Pico chemiluminescence substrate and captured using the ChemiDoc imaging instrument. Primary antibodies used in this study: anti-AHR antibody (BML-SA210 -Enzo Biochem, Farmingdale, NY), anti-p27Kip1 antibody (610242 -BD Biosciences), anti-p53 antibody (DO-1 sc-126 -Santa Cruz Biotechnology, Dallas, TX), anti-p21 antibody (2947S -Cell Signaling Technology, Danvers, MA), and anti-GAPDH antibody (sc-36502, Santa Cruz).
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