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37 fatty acid methyl esters fames

Manufactured by Merck Group
Sourced in United States, United Kingdom

37 fatty acid methyl esters (FAMEs) is a reference standard containing 37 individual fatty acid methyl ester compounds. It serves as a calibration mixture for the identification and quantification of fatty acids in various samples using gas chromatography (GC) analysis.

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2 protocols using 37 fatty acid methyl esters fames

1

Fatty Acid Profiling of Nori and Oils

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Unseasoned dried nori and sample oils were purchased from local grocery stores (Jinju, Korea). To prevent nori quality from influencing the results of this study, one kind of nori was used throughout the study. Upon receipt, oil samples (i.e., camellia oil and sesame oil) were flushed with nitrogen and stored at −40°C to prevent oxidation prior to analysis. HPLC-grade methanol, hexane, boron trifluoride in methanol, anhydrous sodium sulfate, and sodium chloride were purchased from Fisher Scientific (Suwanee, GA, USA). Heptadecanoic acid (98% purity), diethyl ether, potassium hydroxide, 1% phenolphthalein in ethanol (v/v), pentadecane, n-alkanes (C8–C20), and a lipid standard mixture of 37 fatty acid methyl esters (FAMEs) were acquired from Sigma-Aldrich (St. Louis, MO, USA). Other chemicals were of analytical grade.
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2

Metabolite Standards Acquisition Protocol

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Aqueous metabolite standards were purchased form Sigma Aldrich (Poole, Dorset, UK). Lipid standards were purchased form Avanti-Polar lipids (Alabaster, Alabama, US) with the exception of the standard mix containing 37 fatty acid methyl esters (FAMEs) (Sigma Aldrich, Poole, Dorset, UK). Deuterated compounds used as internal standards were purchased from Cambridge Isotope Laboratories (Andover, Massachusetts, US). Solvents used were of HPLC grade or higher. All other compounds, chemicals and solutions used are detailed where relevant.
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