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Avance dpx 500 mhz nmr spectrometer

Manufactured by Bruker
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Sourced in Switzerland

The AVANCE DPX 500 MHz NMR spectrometer is a high-performance nuclear magnetic resonance (NMR) instrument designed for analytical applications. It operates at a magnetic field strength of 500 MHz and is capable of performing various NMR experiments to analyze the structure and composition of chemical samples.

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Lab products found in correlation

Silica gel 60f254 sheets, by Merck Group (1 mentions) Filtermax f3 multi mode microplate reader, by Molecular Devices (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Gcms qp2010, by Shimadzu (1 mentions) Prostar 335, by Agilent Technologies (1 mentions) Unity inova 300 mhznmr spectrometer, by Bruker (1 mentions) Star workstation software, by Agilent Technologies (1 mentions) Rotavapor, by Büchi (1 mentions)

Close spelling variants of this product

Avance DPX-500 NMR spectrometer Avance 500 MHz NMR spectrometer Avance DPX-500 spectrometer DPX 500 NMR spectrometer Avance 500 NMR spectrometer Avance 500 MHz spectrometer 500 MHz NMR spectrometer Avance DPX-500 DPX-500 MHz Avance DPX spectrometer

4 protocols using avance dpx 500 mhz nmr spectrometer

1

Characterization of Phytochemicals via Advanced Analytical Techniques

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Fractionation and isolation of compounds were done by column chromatography using 60–120 mesh silica gels. TLC experiments were carried out using precoated Silica gel 60 F254 sheets (Merck, Darmstadt, Germany). 1D and 2D NMR were recorded with a Bruker AVANCE DPX 500 MHz NMR spectrometer, Switzerland with tetramethylsilane (TMS) as the internal reference. Melting points were measured using BUCHI M-560 capillary melting point apparatus. High-Resolution Electro-Spray Ionization Mass spectra (HR-ESI-MS) were recorded using Waters XEVO G2-Xs QT LC-MS system. FT-IR spectra were recorded in Elmer FT-IR 2000 spectrometer on a thin film using chloroform. A549 Lung cancer and MIAPaCa2 pancreatic cancer cell lines were purchased from NCCS, Pune, India. MTT was purchased from Sigma-Aldrich Co (St Louis, MO, USA). The absorbance was measured on an ELISA plate reader (FilterMax F3 Multi-Mode Microplate Readers, Molecular Devices) with a test wavelength of 570 nm and a reference wavelength of 630 nm.
DUTTA D., NARZARY B., SAIKIA S., TAMULI K.J., BORDOLOI M., LHOUVUM N., NATH S., SAHOO R.K., GOGOI B., SAIKIA S, & BHATTACHARYYA N.K. (2022). Major chemical constituents from Illicium griffithii Hook. f. & Thoms of North East India and their cytotoxicity and antimicrobial activities. Turkish Journal of Chemistry, 46(5), 1468-1476.
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2

Methylation and Analysis of Extracellular Polysaccharides

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A solution of 5 mg of purified EPS in 0.5 mL of DMSO (dimethyl sulfoxide) was methylated by adding finely powdered NaOH (20 mg) and methyl iodide (0.1 mL) prior to sonication for 15 min. The methylated products were extracted with CHCl3 and H2O (5∶2, v/v). The CHCl3 phase was separated and dried under N2 and hydrolyzed in 2 M TFA at 100°C for 6 h. The hydrolyzed EPS was reduced with 50 mM sodium borohydride at room temperature for 4 h, evaporated three times from a mixture of acetic acid/methyl alcohol (1∶1), and acetylated in 50∶50 acetic anhydride/pyridine at 100°C for 90 min. Alditol acetates of the methylated sugars were analyzed using a Shimadzu GCMS-QP2010. NMR spectra were obtained using a Bruker Avance DPX-500 MHz NMR Spectrometer (Bruker Co., Billerica, MA). The proton and 13C spectra were run at a probe temperature of 40°C. The purified samples were dried in a vacuum over P2O5 and then exchanged with deuterium by lyophilizing several times with D2O. The EPS was dissolved in 0.7 mL of D2O (99.96%) at concentrations of 15 mg/mL (1H NMR) and 30 mg/mL (13C NMR). The 2D COSY experiment was recorded at a mixing time of 150 ms.
Roy Chowdhury S., Sengupta S., Biswas S., Sinha T.K., Sen R., Basak R.K., Adhikari B, & Bhattacharyya A. (2014). Bacterial Fucose-Rich Polysaccharide Stabilizes MAPK-Mediated Nrf2/Keap1 Signaling by Directly Scavenging Reactive Oxygen Species during Hydrogen Peroxide-Induced Apoptosis of Human Lung Fibroblast Cells. PLoS ONE, 9(11), e113663.
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3

Characterization of Synthetic Compounds

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Melting points were determined using a MEL-TEMP II capillary melting point apparatus and are uncorrected. Nuclear magnetic resonance (1H NMR and 13C NMR) spectra were obtained on a Varian Avance DPX-500 MHz NMR spectrometer or a Bruker Unity Inova 300 MHz NMR spectrometer. Chemical shifts are reported in parts per million (ppm) with reference to TMS as internal standard. Mass spectra (MS) were determined using a PerkinElmer Sciex AP1 150 EX mass spectrometer equipped with an ESI (turbospray) source. Elemental analyses were performed by Atlantic Microlab Inc., Atlanta, GA. The purity of the compounds (>95%) was established by elemental analysis. Optical rotations were measured on a Rudolf Research AutoPol III polarimeter. Analytical HPLC was performed using a dual pump system (Varian Prostar 210 solvent system delivery system with 5 mL pump heads), a Rheodyne injector and a Varian ProStar 335 diode-array detector (DAD) controlled by Varian Star Workstation software. Unless otherwise stated, reagent-grade chemicals were obtained from commercial sources and were used without purification. All moisture- and airsensitive reactions and reagent transfers were carried out under dry nitrogen.
Chang S.D., Brieaddy L.E., Harvey J.D., Lewin A.H., Mascarella S.W., Seltzman H.H., Reddy P.A., Decker A.M., McElhinny CJ J.r., Zhong D., Peterson E.E., Navarro H.A., Bruchas M.R, & Carroll F.I. (2015). Novel Synthesis and Pharmacological Characterization of NOP Receptor Agonist 8-[(1S,3aS)-2,3,3a,4,5,6-Hexahydro-1H-phenalen-1-yl]-1-phenyl-1,3,8-triazaspiro[4.5]decan-4-one (Ro 64-6198). ACS chemical neuroscience, 6(12), 1956-1964.
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4

NMR, Melting Point, and Mass Spectrometry Analysis

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1 H-and 13 C-NMR were recorded with a Bruker AVANCE DPX 500 MHz NMR spectrometer, Switzerland with internal reference as tetramethylsilane (TMS). Chemical shifts (δ) value are reported in ppm and coupling constant (J) in Hz. Melting point was performed using BUCHI M-560 capillary melting point apparatus and is uncorrected. A high resolution mass spectrum (HR-ESI-MS) was recorded in a Waters XEVO G2-Xs QT instrument. A FT-IR spectrum in Elmer FT-IR 2000 spectrometer on a thin film using chloroform was recorded. Silica gel 60-120 mesh was used for column chromatography. Thin layer chromatography (TLC) experiments were performed using pre-coated silica gel 60 F254 sheets (Merck, Darmstadt, Germany). Fractionated solvents from column chromatography were evaporated to dryness under reduced pressure by Rotavapor; BUCHI, Labortechnik AG, Flawil, Switzerland. The microorganisms Escherichia coli (ATCC®11229TM) and Candida albicans (ATCC®90028TM) for antimicrobial assay were purchased from HiMedia.
Dutta D., Bordoloi M.J., & Bhattacharyya N.K. (2021). Antimicrobial Compound Isolated From The Plant Pothos scandens L. Egyptian Journal of Chemistry, 0(0), 0-0.
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