Hemoccult kits

Body weight, stool consistency and intestinal bleeding were determined up to 10 days after 2% DSS administration. Scoring for stool consistency and intestinal bleeding was accessed as described previously 27 (link). In a brief, stool consistency scores were determined as followed: well-formed stools were scored as 0, semi-formed stools which didn't adhere to the anus were scored as 1, semi-formed stools adhering to the anus were scored as 2, and diarrhea was scored as 3. Intestinal bleeding scores were determined as followed: no blood determined by using hemoccult kits (Nanjing Jiancheng bioengineering Institute, Nanjing, China) was scored as 0, positive hemoccult was scored as 1, visible blood traces in stool was scored as 2, and rectal bleeding was scored as 3. The averaged stool consistency and intestinal bleeding scores were calculated as the clinical score.

Dextran sulfate sodium (DSS, molecular weight 36–50 kDa) was bought from MP Biomedical Corporation (Santa Ana, California, USA). Carboxyl methyl cellulose-Na (CMC-Na) was gained from Shanghai Changguang Enterprise Development Co., Ltd. (Shanghai, China). AIN93G purified rodent diet was acquired from Jiangsu Xietong Pharmaceutical Bioengineering Co., Ltd. (Jiangsu, China). Hemoccult Kits, MPO test kits, BCA test kits were received from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Antibodies for NF-κB p65, IκB-α and phosphor-IκB-α were obtained from Thermo Scientific Co., Ltd. (Waltham, MA, USA). Trizol reagents were acquired from Invitrogen (Carlsbad, CA, USA). Revert Aid reverse transcription kits, PCR SuperMix, RNase-Free Water were purchased from Thermo Scientific Co., Ltd. (Waltham, MA, USA).