1 fluoro 2 4 dinitrobenzene dnfb d1529

Manufactured by Merck Group

1-Fluoro-2,4-dinitrobenzene (DNFB, D1529) is a chemical compound commonly used as a reagent in various laboratory applications. It is a pale yellow crystalline solid with a distinct odor. The compound has specific chemical properties that make it useful for certain research and analytical purposes.

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Lab products found in correlation

Pen strep, by Merck Group (2 mentions) Sodium pyruvate, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions) L glutamine, by Merck Group (2 mentions) Blasticidin, by Merck Group (2 mentions) Rmpi 1640, by Thermo Fisher Scientific (2 mentions) Calu 1, by American Type Culture Collection (2 mentions) Sk mes 1, by American Type Culture Collection (2 mentions) Lenticas9 blast, by Addgene (2 mentions) Rmpi 1640, by Merck Group (2 mentions) Beas 2b, by American Type Culture Collection (1 mentions)

Spelling variants of this product

4-dinitrobenzene (8 citations)

2 protocols using 1 fluoro 2 4 dinitrobenzene dnfb d1529

Cell Culture Conditions and Reagents

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All cell lines were cultured in media supplemented with 10% FBS, 1% Pen/Strep and 1% L-Glutamine (all from Sigma) at 37 °C and 5% CO₂ in a humidified incubator. H460, H1299, H520, SK-MES-1 and Calu-1 (from ATCC) were cultured in RMPI-1640 (Sigma). H23 and H838 (both from ATCC) cells were cultured in RMPI-1640, supplemented with 1 mM sodium pyruvate (Gibco). A549, 293T, BEAS-2B (ATCC), BEN (DSMZ), murine Lewis lung carcinoma cell line LL2, Ladi2.1 and Ladi3.1 cells (derived from p53fl/fl-LSL KRASG12D/ + mouse NSCLC model) were cultured in DMEM (Sigma). Human cells were STR-profiled, used between passages 3 and 15, examined for mycoplasma regularly (detection kit from Invivogen). The A549-Cas9 cell line was generated by lentiviral transduction with lentiCas9-Blast (Addgene #52962) and selection with 100 μg/mL of blasticidin (Sigma) for 3 days. KHS101 hydrochloride (4888) was purchased from Tocris; cisplatin (CAS 15663–27-1), Z-VAD-FKM (CAS 187389–52-2) and 2-Deoxy-D-Glucose (2-DG, sc-202010) were purchased from Santa Cruz Biotechnology; Necrostatin-1 (N9037-10NMG), Ferrostatin-1 (SML0583-5MG) and 1-Fluoro-2,4-dinitrobenzene (DNFB, D1529) were purchased from Sigma. HB072 was obtained as previously shown [23 ].

Parma B., Ramesh V., Gollavilli P.N., Siddiqui A., Pinna L., Schwab A., Marschall S., Zhang S., Pilarsky C., Napoli F., Volante M., Urbanczyk S., Mielenz D., Schrøder H.D., Stemmler M., Wurdak H, & Ceppi P. (2021). Metabolic impairment of non-small cell lung cancers by mitochondrial HSPD1 targeting. Journal of Experimental & Clinical Cancer Research : CR, 40, 248.

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Cell Culture Protocols for NSCLC Studies

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All cell lines were cultured in media supplemented with 10% FBS, 1% Pen/Strep and 1% L-Glutamine (all from Sigma) at 37 °C and 5% CO2 in a humidified incubator. H460, H1299, H520, SK-MES-1 and Calu-1 (from ATCC) were cultured in RMPI-1640 (Sigma). H23 and H838 (both from ATCC) cells were cultured in RMPI-1640, supplemented with 1 mM sodium pyruvate (Gibco). A549 (ATCC), BEN (DSMZ), murine Lewis lung carcinoma cell line LL2, Ladi2.1 and Ladi3.1 cells (derived from p53fl/fl-LSL KRASG12D/+ mouse NSCLC model) were cultured in DMEM (Sigma). Human cells were STR-profiled, used between passages 3 and 15, examined for mycoplasma regularly (detection kit from Invivogen). A549-Cas9 cell line was generated by lentiviral transduction with lentiCas9-Blast (Addgene #52962) and selection with 100 μg/mL of blasticidin (Sigma) for 3 days. KHS101 hydrochloride (4888) was purchased from Tocris; cisplatin (CAS 15663-27-1), Z-VAD-FKM (CAS 187389-52-2) and 2-Deoxy-D-Glucose (sc-202010) were purchased from Santa Cruz Biotechnology; Adenosine 5′-triphosphate (ATP) disodium salt hydrate (A1852-1VL), Necrostatin-1 (N9037-10NMG), Ferrostatin-1 (SML0583-5MG) and 1-Fluoro-2,4-dinitrobenzene (DNFB, D1529) were purchased from Sigma. HB072 was obtained as previously shown (23) .

Parma B., Ramesh V., Gollavilli P.N., Siddiqui A., Pinna L., Schwab A., Marschall S., Zhang S., Pilarsky C., Napoli F., Volante M., Urbanczyk S., Mielenz D., Schrøder H.D., Stemmler M.P., Wurdak H., & Ceppi P. (2021). Metabolic breakdown of non-small cell lung cancers by mitochondrial HSPD1 targeting.

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