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Ab240087

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Ab240087 is a laboratory equipment product. It is a component designed for use in scientific research applications.

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Lab products found in correlation

Ab95572, by Abcam (1 mentions) Fitc annexin 5 apoptosis detection kit 1, by BD (1 mentions) Ab80826, by Abcam (1 mentions) Picogreen, by Yeasen (1 mentions)

2 protocols using ab240087

1

Apoptosis and MHC-I Assessment

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Cells were treated with drugs for 48 hours and then stained with an FITC Annexin V Apoptosis Detection Kit I (556547, BD Biosciences). Apoptosis was measured by Flow Cytometer (Beckman Coulter). The cells were incubated with anti–MHC‐I (ab240087, Abcam) (ab95572, Abcam) for 1 hour. Next, the cells were incubated with secondary antibody for 1 hour. The samples were detected by flow cytometry and quantified with FlowJo‐V10 software.
Wu X., Kang X., Zhang X., Xie W., Su Y., Liu X., Guo L., Guo E., Li F., Hu D., Qin X., Fu Y., Peng W., Jia J, & Wang C. (2021). WEE1 inhibitor and ataxia telangiectasia and RAD3‐related inhibitor trigger stimulator of interferon gene‐dependent immune response and enhance tumor treatment efficacy through programmed death‐ligand 1 blockade. Cancer Science, 112(11), 4444-4456.
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2

Immunofluorescence Assay for Cellular Responses

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After treatment with drugs for 72 hours, 3 µg/mL PicoGreen (12641ES01, Yeasen) was added to the medium. After 1 hour, cells were fixed (4% paraformaldehyde), permeabilized (0.25% TritonX‐100) and blocked (5% BSA). Cells were then stained with anti–pan Cytokeratin (ab80826, Abcam), phospho‐Histone H2A.X (9718, CST), phosphor‐TBK1 (5483, CST), phospho‐IRF‐3 (29047, CST), and anti–MHC‐I (ab240087, Abcam) (ab281902, Abcam) overnight at 4°C. Cells were stained with secondary antibody for 1 hour and then counterstained with DAPI. Photographs were taken using confocal microscopy.
Wu X., Kang X., Zhang X., Xie W., Su Y., Liu X., Guo L., Guo E., Li F., Hu D., Qin X., Fu Y., Peng W., Jia J, & Wang C. (2021). WEE1 inhibitor and ataxia telangiectasia and RAD3‐related inhibitor trigger stimulator of interferon gene‐dependent immune response and enhance tumor treatment efficacy through programmed death‐ligand 1 blockade. Cancer Science, 112(11), 4444-4456.
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