Amino acid derivatives and resins

Amino acid derivatives and resins were obtained from Iris Biotech (Marktredwitz, Germany). Reagents, such as N,N′-diisopropylcarbodiimide (DIC), triisopropylsilane (TIS), 1-hydroxybenzotriazole (HOBt), 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), Isoniazid (INH), glyoxylic acid and 5(6)-carboxyfluorescein (Cf) were purchased from Sigma (Budapest, Hungary). Trifluoroacetic acid (TFA) and acetonitrile (AcN) were from VWR (Budapest, Hungary). N,N-dimethylformamide (DMF), dichlormethane (DCM), diethyl ether, and ethanol were purchased from Reanal (Budapest, Hungary)
For the in vitro assays, RPMI-1640 medium, fetal calf serum (FCS), and trypan blue were obtained from Sigma (Budapest, Hungary), while DMEM medium and 2 mM l-glutamine were from Lonza (Basel, Switzerland). Trypsin, nonessential amino acids, and penicillin-streptomycin were from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). HPMI buffer (9 mM glucose, 10 mM NaHCO₃, 119 mM NaCl, 9 mM HEPES, 5 mM KCl, 0.85 mM MgCl₂, 0.053 mM CaCl₂, 5 mM Na₂HPO₄ × 2H₂O, pH = 7.4) was prepared in-house using components obtained from Sigma (Budapest, Hungary). BBL Löwenstein-Jensen medium was from Becton Dickinson (Környe, Hungary).