Drugs were incubated 2 h prior to imaging unless stated otherwise. The drug concentrations are as follow: (S)-nitro-blebbistatin (Cayman Chemical) 50 μM, NSC-23766 (Sigma–Aldrich) 200 µM, SMIFH2 (Sigma) 50 μM dissolved in DMSO. Caspase-3 indicator DEVD-FMK conjugated to Sulfo-rhodamine (Abcam) was used at 1:1000 dilution and incubated with the cells 30 min before experiment. To visualize F-actin in certain experiments, siR-actin (Cytoskeleton) was added at 100 nM concentration to the medium 12 h before imaging. Hoechst 33342 at 1 µg/mL (Sigma) was added into culture media 1 h and washed before imaging for nucleus live detection.
Devd fmk conjugated to sulfo rhodamine
Manufactured by Abcam
DEVD-FMK conjugated to Sulfo-rhodamine is a fluorescent caspase-3 inhibitor. It is used to label and detect active caspase-3 in cells undergoing apoptosis.
Automatically generated - may contain errors
2 protocols using devd fmk conjugated to sulfo rhodamine
1
Live-Cell Imaging of Cytoskeletal Dynamics
2
Multimodal Imaging of Actin Dynamics
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Drugs were incubated 2 hours prior to imaging unless stated otherwise. The drug concentrations are as follow: (S)-nitro-blebbistatin (Cayman Chemical) 50 μM, NSC-23766 (Sigma-Aldrich) 200 µM, SMIFH2 (Sigma) 50 μM dissolved in DMSO. Caspase-3 indicator DEVD-FMK conjugated to Sulfo-rhodamine (Abcam) was used at 1:1000 dilution and incubated with the cells 30 min before experiment. To visualize F-actin in certain experiments, siR-actin (Cytoskeleton) was added at 100 nM concentration to the medium 12 hours before imaging. Hoechst 33342 at 1µg/mL (Sigma) was added into culture media 1 hour and washed before imaging for nucleus live detection.
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