Atm inhibitor ku55933

Camptothecin (CPT), Cisplatin (CDDP), Topotecan, ATM inhibitor (KU55933), and WDR5 inhibitor (OICR-9429) PRMT5 inhibitor (GSK591) were purchased from MedChemexpress (Monmouth Junction, NJ, USA). Human recombinant His-JDP2 protein was purchased from Abcam (Cambridge, MA, USA).

HeLa, SiHa, T24, and 293T cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Carlsbad, CA, United States of America), supplemented with 10% (v/v) fetal bovine serum (Biological Industries). HCT116, HCT116 (p53-/-), H1299, and SW620 were maintained in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) supplemented with the 10% FBS. HITT sequence was subcloned into a lent-virus vector, pLnc-KP. Virus was packaged in HEK-293T cells and then infected HCT116 and HeLa cells. Single clones were selected by puromycin and the selected clones with HITT overexpressed similar to the normal cells were used to do further experiments. DR-U2OS, EJ2-U2OS, and EJ5-U2OS were kindly provided by Prof. Jeremy M. Stark [35 (link)]. All cells were grown at 37°C in the humidified incubator (Thermo Scientific) with 5% CO₂. Cell lines were routinely tested to exclude mycoplasma contamination, and all cells have never been passaged longer than 1 mo. All drugs used in this study were as follows: Doxorubicin (Selleck, #S1208); Calicheamicin (MedChemExpress, #HY-19609); Bleomycin (Apexbio, #A8331); Etoposide (Apexbio, A1971); ATM inhibitor Ku60019 (Apexbio, #A8336); ATM inhibitor Ku55933 (MedChemExpress, #HY-12016); and Actinomycin D (MedChemExpress, #HY-17559).