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Mouse antibody against cyclin d1

Manufactured by Santa Cruz Biotechnology
Sourced in United States

This Mouse antibody against Cyclin D1 is a laboratory reagent designed for the detection and analysis of Cyclin D1 protein expression. Cyclin D1 is a key regulator of the cell cycle and its expression is often altered in various disease states. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to study Cyclin D1 in biological samples.

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2 protocols using mouse antibody against cyclin d1

1

Antibody Procurement for Protein Analysis

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Roswell Park Memorial Institute 1640 medium was purchased from Thermo Fisher Scientific (Waltham, MA). F‐12K medium was purchased from Sigma‐Aldrich (St Louis, MO). The rabbit antibody against c‐MYC was purchased from Abcam (Cambridge, UK). The rabbit antibody against CPA4 was purchased from Genetex (Irvine, CA). The rabbit antibodies against α‐tubulin, β‐actin, P27, cyclin‐dependent kinase 4 (CDK4), B‐cell lymphoma‐2 (Bcl‐2), caspase 9, caspase 3, p‐AKT, and AKT and the mouse antibody against flag were purchased from Cell Signaling Technology (Beverly, MA). The mouse antibody against Cyclin D1 was purchased from Santa Cruz Biotechnology (Santa Cruz, MO).
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2

Molecular Markers for Cancer Signaling

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The EGCG (Mw 458.4, purity ≥95%; Sigma-Aldrich, St. Louis, USA) was dissolved in water for storage at 2-8°C. Bortezomib (Velcade; Millennium Predictive Medicine, Inc., Cambridge, USA) was dissolved in saline at 10 mM for storage at -20°C. Rabbit antibody against β-catenin, mouse antibody against cyclin D1, mouse antibody against c-Myc, mouse antibody against β-actin, and fluorescein isothiocyanate (FITC)-conjugated secondary antibody were all purchased from Santa Cruz Biotechnology (Santa Cruz, USA).
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