Stealth select rnai sirna nm 000567

Eight-plex iTRAQ reagent kits were acquired from Applied Biosystems (Foster City, CA, USA). Monoclonal antibodies against human CRP were obtained from HyTest (Finland, Turku). Monoclonal antibodies against UGDH, EphB3, ENO2, ANXA2, KRT5, MSH2, SHC1, HSP90B1, MAPK, p-MAPK, ERK, p-ERK and HIF1α were purchased from Abcam (Cambridge, USA). CRP speci c Stealth Select RNAi™ siRNA (NM_000567), Stealth RNAi™ Negative Control siRNA and Lipofectamine Max transfection reagent were obtained from Santa Cruz (California, USA). Small interfering RNA against human EphB3 (ID 146498, ID 146499) were purchased from Invitrogen (Grand Island, NY). IP lysis buffer was purchased from Beyotime (Shanghai, China). Protein A/G agarose beads from GE Healthcare (Little Chalfont, UK). CytoSelect™ 24-Well Cell Migration and Invasion Assay kits (8 µm, colorimetric format) were purchased from Cell Biolabs (San Diego, CA, USA).

Eight-plex iTRAQ reagent kits were acquired from App lied Biosystems (Foster City, CA, USA). Monoclonal antibodies against human CRP were obtained from HyTest (Finland, Turku). Monoclonal antibodies against UGDH, EphB3, ENO2, ANXA2, KRT5, MSH2, SHC1, HSP90B1, MAPK, p-MAPK, ERK, p-ERK and HIF1α were purchased from Abcam (Cambridge, USA). CRP specific Stealth Select RNAi™ siRNA (NM_000567), Stealth RNAi™ Negative Control siRNA and Lipofectamine Max transfection reagent were obtained from Santa Cruz (California, USA). Small interfering RNA against human EphB3 (ID 146498, ID 146499) were purchased from Invitrogen (Grand Island, NY). IP lysis buffer was purchased from Beyotime (Shanghai, China). Protein A/G agarose beads from GE Healthcare (Little Chalfont, UK). CytoSelect™ 24-Well Cell Migration and Invasion Assay kits (8 μm, colorimetric format) were purchased from Cell Biolabs (San Diego, CA, USA).