1 2 dioleoyl sn glycero 3 phosphate sodium salt dopa

1-Oleoyl-rac-glycerol and Pluronic F127 were purchased from Sigma Aldrich. Additional lipids (ovine cholesterol (CHOL), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phosphate (sodium salt) (DOPA)) were purchased from Avanti Polar Lipids. All lipids were used without further purification and placed on a lyophilser and dried for a minimum of 24 h before usage. They were subsequently weighed individually into glass vials and dissolved in chloroform to prepare lipid stock solutions of up to a maximum concentration of 20 mg mL⁻¹. Lipid films were prepared in glass vials using Hamilton syringes to transfer the appropriate volumes of lipid stock solutions to obtain the desired molar ratios (55 mol% MO, 30 mol% CHOL, varying ratios of DOPA and DOPC (DOPC +DOPA mol%=15) which were then mixed and subsequently dried under a stream of nitrogen and placed overnight on a lyophilizer. Films were stored under nitrogen at −20 °C until required.