Thiol-modified DNA oligonucleotides (Integrated DNA Technologies) were treated with tris(2-carboxyethyl)phosphine (Sigma-Aldrich) for 30 minutes (50 nmole DNA per reaction) and immediately reacted with 1 µmole of 18:1 MPB PE (Avanti Polar Lipids) in aqueous solutions containing ~2% Octyl β-D-glucopyranoside (OG, purchased from EMD Millipore) at room temperature for 30 minutes. Such a reaction mixture was then diluted ~3-fold with OG concentration brought up to 4%, added to 2.5 µmole of pre-dried DOPC, agitated, and dialyzed overnight. The conjugation products were separated from unconjugated DNA via isopycnic centrifugation in iodixanol gradients (Cosmo Bio USA) and later analyzed by SDS-polyacrylamide gel electrophoresis.
Mpb pe
Manufactured by Avanti Polar Lipids
The MPB PE is a laboratory instrument designed for the purification and analysis of phospholipids. It utilizes high-performance liquid chromatography (HPLC) technology to separate and quantify various phospholipid species present in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Tris 2 carboxyethyl phosphine, by Merck Group (1 mentions)
Thiol modified dna oligonucleotides, by Integrated DNA Technologies (1 mentions)
Octyl β d glucopyranoside og, by Merck Group (1 mentions)
Iodixanol gradients, by Cosmo Bio (1 mentions)
1 2 dioleoyl sn glycero 3 phosphocholine dopc, by Avanti Polar Lipids (1 mentions)
Atto 647n pe, by ATTO-TEC (1 mentions)
Dehydroergosterol dhe, by Merck Group (1 mentions)
Nbd pe, by Avanti Polar Lipids (1 mentions)
Dogs nta ni2, by Avanti Polar Lipids (1 mentions)
Rhod pe, by Avanti Polar Lipids (1 mentions)
3 protocols using mpb pe
1
Lipid-Oligonucleotide Conjugation Protocol
2
Lipid Reagents for Membrane Studies
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1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), DOGS–NTA–Ni2+, Rhod–PE, and MPB–PE were purchased from Avanti Polar Lipids. ATTO647N-PE was purchased from ATTO-TEC.
3
Lipidomics protocol for lipid species
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DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine), liver PI (L-α-phosphatidylinositol, bovine), soy PI, brain PI(4)P (L-α-phosphatidylinositol 4-phosphate), NBD-PE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2–1,3-benzoxadiazol-4-yl)), Rhod-PE (1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl)), DOGS-NTA-Ni2+ (1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] (nickel salt)), MPB-PE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[4-(p-maleimidophenyl) butyramide]) and DNS-PE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(5-dimethylamino-1-naphthalenesulfonyl)) were purchased from Avanti Polar Lipids. Dehydroergosterol (DHE) were from Sigma Aldrich. The concentration of DHE in stock solution in methanol was determined by UV-spectroscopy using an extinction coefficient of 13,000 M−1.cm−1.
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