Adult worms were picked into S-basal buffer and lysed by addition of SDS sample buffer, followed by boiling for 15 min with occasional vortexing. Whole worm lysates were then separated on 4–12% polyacrylamide gradient gels (GenScript, #M00654), transferred to membranes, and blotted with mouse anti-HA (1:1000, Thermo Fisher, #26183), Guinea pig anti-HTP-3 (1:1500, MacQueen et al., 2005 (link)), mouse anti-α-tubulin (1:5000, Millipore Sigma, #05-829), rabbit anti-β-tubulin (1:2000, Abcam, ab6046), or HRP-conjugated anti-ALFA sdAb (1:1000, Nanotag Biotechnologies, N1505-HRP), HRP-conjugated anti-mouse secondary antibodies (Jackson Immunoresearch #115-035-068), HRP-conjugated anti-Rabbit secondary antibodies (Jackson Immunoresearch #111-035-144), HRP-conjugated anti-Guinea pig secondary antibodies (Jackson Immunoresearch #106-035-003). SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher, #34095) was used for detection.
Guinea pig anti htp 3
Manufactured by Merck Group
Guinea pig anti-HTP-3 is a laboratory reagent used in research applications. It is an antibody raised in guinea pigs that specifically binds to the HTP-3 protein. The core function of this product is to facilitate the detection and study of the HTP-3 protein in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti β tubulin, by Abcam (2 mentions)
Hrp conjugated anti rabbit secondary antibody, by Jackson ImmunoResearch (2 mentions)
Hrp conjugated anti mouse secondary antibody, by Jackson ImmunoResearch (2 mentions)
Mouse anti ha, by Thermo Fisher Scientific (2 mentions)
Hrp conjugated anti guinea pig secondary antibodies, by Jackson ImmunoResearch (2 mentions)
Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (2 mentions)
Mouse anti α tubulin, by Merck Group (2 mentions)
2 protocols using guinea pig anti htp 3
1
Worm Protein Extraction and Western Blot
2
Worm Protein Lysate Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Adult worms were picked into S-basal buffer and lysed by addition of SDS sample buffer, followed by boiling for 15 min with occasional vortexing. Whole worm lysates were then separated on 4-12% polyacrylamide gradient gels (GenScript, #M00654), transferred to membranes, and blotted with mouse anti-HA (1:1,000, Thermo Fisher, #26183), Guinea pig anti-HTP-3 [1:1,500, (MacQueen et al., 2005) (link)], mouse anti-α-tubulin (1:5,000, Millipore Sigma, #05-829), rabbit anti-β-tubulin (1:2,000, Abcam, ab6046), or HRPconjugated anti-ALFA sdAb (1:1,000, Nanotag Biotechnologies, N1505-HRP), HRPconjugated anti-mouse secondary antibodies (Jackson Immunoresearch #115-035-068), HRP-conjugated anti-Rabbit secondary antibodies (Jackson Immunoresearch #111-035-144), HRP-conjugated anti-Guinea pig secondary antibodies (Jackson Immunoresearch #106-035-003). SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher, #34095) were used for detection.
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