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Mouse monoclonal anti cnp ase ab

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The Mouse monoclonal anti-CNP-ase Ab is a laboratory research tool that detects the presence of 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP-ase), an enzyme found in the myelin sheath of the central nervous system. This antibody can be used to identify and quantify CNP-ase in various experimental settings.

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Lab products found in correlation

Mouse monoclonal anti mbp ab, by US Biological (2 mentions) Anti β actin peroxidase conjugated antibody, by Merck Group (2 mentions)

2 protocols using mouse monoclonal anti cnp ase ab

1

Quantitative Analysis of Myelin Proteins

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To exclude potential discrepancies in cerebral sampling which can affect myelin content, the entire single hemisphere was used for quantitation of MBP and CNP-ase, detected with mouse monoclonal anti-MBP Ab (1:1000, #M9758–01, United States Biological, Salem, MA) and mouse monoclonal anti-CNP-ase Ab (1:10,000, #C-5922, Millipore Sigma, St. Louis, MO). Anti-β-Actin peroxidase-conjugated antibody (1:100,000, #A3854, Millipore Sigma, St. Louis, MO) was used for control of loading. Images of the blots were obtained using Fluorchem M Western Imaging System (Protein Simple, San Jose, CA) and processed with Image J software. The results were expressed as optical density ratios between the protein-specific and β-actin bands.
Sosunov S.A., Niatsetskaya Z.V., Stepanova A.A., Galkin A.S., Juliano C.E., Ratner V.I, & Ten V.S. (2021). Developmental window of vulnerability to white matter injury driven by sublethal intermittent hypoxemia. Pediatric research, 91(6), 1383-1390.
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2

Quantitative Analysis of Myelin Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
To exclude potential discrepancies in cerebral sampling which can affect myelin content, the entire single hemisphere was used for quantitation of MBP and CNP-ase, detected with mouse monoclonal anti-MBP Ab (1:1000, #M9758–01, United States Biological, Salem, MA) and mouse monoclonal anti-CNP-ase Ab (1:10,000, #C-5922, Millipore Sigma, St. Louis, MO). Anti-β-Actin peroxidase-conjugated antibody (1:100,000, #A3854, Millipore Sigma, St. Louis, MO) was used for control of loading. Images of the blots were obtained using Fluorchem M Western Imaging System (Protein Simple, San Jose, CA) and processed with Image J software. The results were expressed as optical density ratios between the protein-specific and β-actin bands.
Sosunov S.A., Niatsetskaya Z.V., Stepanova A.A., Galkin A.S., Juliano C.E., Ratner V.I, & Ten V.S. (2021). Developmental window of vulnerability to white matter injury driven by sublethal intermittent hypoxemia. Pediatric research, 91(6), 1383-1390.
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