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V 1 grade

Manufactured by SPI Supplies

The V-1 grade lab equipment is a versatile tool designed for general laboratory applications. It serves as a reliable and accurate instrument for various measurements and procedures within the controlled laboratory environment.

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4 protocols using v 1 grade

1

Structural Analysis of ProMMP-7 Complexes

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ProMMP-7-E195A and truncated forms were prepared at 50 or 100 nM in imaging buffer (10 mM CaCl2, pH 7.5; see SI). Mixtures with Hep dp8 or dp16, heparin, HS, or HS dp8 were examined. Each solution was deposited immediately after mixing on a freshly cleaved muscovite mica surface (V-1 grade, Spi Supplies) and incubated for ~5 min. The surface was then rinsed three times with ~100 µL of imaging buffer. AFM images were acquired in imaging buffer in tapping mode using a commercial instrument (Cypher, Asylum Research). SNL tips (sharp nitride levers, SNL-10, Bruker) with measured spring constants of ~0.2 N/m were used. Images were recorded at ~30°C with an estimated tip-sample force < 100 pN, deduced by comparing the free space tapping amplitude (~5 nm) to the imaging set point amplitude. Under such conditions, minimal protein distortion is expected. Image analysis is detailed in SI.
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2

Atomic Force Microscopy of Silver Nanoparticles

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Atomic force microscopy (AFM) studies were performed to determine the size and surface morphology of synthesized AgNPs on a "Solver Pro" microscope (NT-MDT Spectrum Instruments, Moscow, Russia), equipped with an optical microscope. Measurements were carried out in tapping mode using AFM probes RTESPA (Bruker, Mannheim, Germany, 300 kHz, 40 N/m). A freshly cleaved atomically smooth surface of mica (V1 grade, SPI Supplies) was used as a substrate. A droplet of suspension (0.2 µL) containing nanoparticles (naked AgNPs, AgNP-TBA, and AgNP-MUA) was applied onto a limited area of the mica and left until complete evaporation of the water. The optimal measurement concentrations of AgNPs were achieved by a dilution of stock solutions with distilled water.
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3

AFM Visualization of C60 in Aqueous Solution

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The state of C60 fullerene in aqueous solution was monitored using atomic force microscopy (AFM; ‘Solver Pro M’ system; NT-MDT, Russia). The samples were deposited by precipitation from an aqueous solution droplet onto cleaved mica substrate (V-1 Grade, SPI Supplies). Measurements were performed after complete evaporation of the solvent. The sample visualization was carried out in semicontact (tapping) mode with NSG10 (NT-MDT) probes.
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4

Visualizing WT MT1-MMP Ectodomain on Mica

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Following recent work (Fulcher et al., 2014 (link)), WT MT1-MMP ectodomain was deposited at 100 nM in imaging buffer of 20 mM Tris·HCl (pH 7.5), 10 mM CaCl2, and 150 mM NaCl on freshly cleaved muscovite mica surface (V-1 grade, SPI Supplies), incubated for 1 min, rinsed three times with ~100 μL of the imaging buffer, and incubated another 5 min. Images were acquired at room temperature in the imaging buffer using tapping mode and a Cypher instrument (Asylum Research). Biolever mini tips (BL-AC40TS, Olympus) with spring constants ~0.06 N/m were used. Images were recorded with an estimated tip-sample force <100 pN for minimal distortion of proteins.
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