V 1 grade

Manufactured by SPI Supplies

The V-1 grade lab equipment is a versatile tool designed for general laboratory applications. It serves as a reliable and accurate instrument for various measurements and procedures within the controlled laboratory environment.

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Lab products found in correlation

Snl 10, by Bruker (1 mentions) Rtespa, by Bruker (1 mentions) Nsg 10, by NT-MDT (1 mentions) Solver pro m system, by NT-MDT (1 mentions) Cypher instrument, by Oxford Instruments (1 mentions)

4 protocols using v 1 grade

Structural Analysis of ProMMP-7 Complexes

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ProMMP-7-E195A and truncated forms were prepared at 50 or 100 nM in imaging buffer (10 mM CaCl₂, pH 7.5; see SI). Mixtures with Hep dp8 or dp16, heparin, HS, or HS dp8 were examined. Each solution was deposited immediately after mixing on a freshly cleaved muscovite mica surface (V-1 grade, Spi Supplies) and incubated for ~5 min. The surface was then rinsed three times with ~100 µL of imaging buffer. AFM images were acquired in imaging buffer in tapping mode using a commercial instrument (Cypher, Asylum Research). SNL tips (sharp nitride levers, SNL-10, Bruker) with measured spring constants of ~0.2 N/m were used. Images were recorded at ~30°C with an estimated tip-sample force < 100 pN, deduced by comparing the free space tapping amplitude (~5 nm) to the imaging set point amplitude. Under such conditions, minimal protein distortion is expected. Image analysis is detailed in SI.

Fulcher Y.G., Gari R.R., Frey N.C., Zhang F., Linhardt R.J., King G.M, & Van Doren S.R. (2014). Heparinoids activate a protease, secreted by mucosa and tumors, via tethering supplemented by allostery. ACS chemical biology, 9(4), 957-966.

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Atomic Force Microscopy of Silver Nanoparticles

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Atomic force microscopy (AFM) studies were performed to determine the size and surface morphology of synthesized AgNPs on a "Solver Pro" microscope (NT-MDT Spectrum Instruments, Moscow, Russia), equipped with an optical microscope. Measurements were carried out in tapping mode using AFM probes RTESPA (Bruker, Mannheim, Germany, 300 kHz, 40 N/m). A freshly cleaved atomically smooth surface of mica (V1 grade, SPI Supplies) was used as a substrate. A droplet of suspension (0.2 µL) containing nanoparticles (naked AgNPs, AgNP-TBA, and AgNP-MUA) was applied onto a limited area of the mica and left until complete evaporation of the water. The optimal measurement concentrations of AgNPs were achieved by a dilution of stock solutions with distilled water.

Borowik A., Butowska K., Konkel K., Banasiuk R., Derewonko N., Wyrzykowski D., Davydenko M., Cherepanov V., Styopkin V., Prylutskyy Y., Pohl P., Krolicka A, & Piosik J. (2019). The Impact of Surface Functionalization on the Biophysical Properties of Silver Nanoparticles. Nanomaterials, 9(7), 973.

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AFM Visualization of C60 in Aqueous Solution

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The state of C₆₀ fullerene in aqueous solution was monitored using atomic force microscopy (AFM; ‘Solver Pro M’ system; NT-MDT, Russia). The samples were deposited by precipitation from an aqueous solution droplet onto cleaved mica substrate (V-1 Grade, SPI Supplies). Measurements were performed after complete evaporation of the solvent. The sample visualization was carried out in semicontact (tapping) mode with NSG10 (NT-MDT) probes.

Prylutska S., Grynyuk I., Matyshevska O., Prylutskyy Y., Evstigneev M., Scharff P, & Ritter U. (2014). C60 Fullerene as Synergistic Agent in Tumor-Inhibitory Doxorubicin Treatment. Drugs in R&D, 14(4), 333-340.

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Visualizing WT MT1-MMP Ectodomain on Mica

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Following recent work (Fulcher et al., 2014 (link)), WT MT1-MMP ectodomain was deposited at 100 nM in imaging buffer of 20 mM Tris·HCl (pH 7.5), 10 mM CaCl₂, and 150 mM NaCl on freshly cleaved muscovite mica surface (V-1 grade, SPI Supplies), incubated for 1 min, rinsed three times with ~100 μL of the imaging buffer, and incubated another 5 min. Images were acquired at room temperature in the imaging buffer using tapping mode and a Cypher instrument (Asylum Research). Biolever mini tips (BL-AC40TS, Olympus) with spring constants ~0.06 N/m were used. Images were recorded with an estimated tip-sample force <100 pN for minimal distortion of proteins.

Zhao Y., Marcink T.C., Gari R.R., Marsh B.P., King G.M., Stawikowska R., Fields G.B, & Van Doren S.R. (2015). Transient Collagen Triple-Helix Binding to a Key Metalloproteinase in Invasion and Development. Structure (London, England : 1993), 23(2), 257-269.

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