Omniplex d neural data acquisition system recording system

Experiments were performed in a custom-built sound-attenuating anechoic chamber. Anesthetized bats were placed in a body mold made of soft plastic foam and the head was tightly affixed to the stereotaxic apparatus by a rod attached to a metal holder. Neuronal recordings were performed using silicon probes from Cambridge Neurotech (16 contacts × 2 shanks per probe with 250 μm between shanks and 50-μm spacing between contact sites along each shank). Each shank had a thickness of 15 μm. Using a micromanipulator system (MX7600R, Siskiyou Corp.), probes were positioned perpendicular to the pial surface based on landmarks and stereotaxic coordinates, and then inserted slowly into the brain through the intact dura mater to a depth of ∼900 ± 50 μm at the deepest contact point. Neuronal data were acquired with an OmniPlex D. Neural Data Acquisition System recording system (Plexon Inc.) at a sampling rate of 40 kHz (per channel) and 16-bit precision. Synchronization between the neural recordings and acoustic stimulus broadcasts was achieved with a TTL pulse output from the National Instrument card and recorded on one of the analog channels of the Plexon data acquisition system.

Experiments were performed in a custom-built sound-attenuating anechoic chamber. Slightly anesthetized bats were placed in a body mold made of soft plastic foam, and the head was tightly affixed to the stereotaxic apparatus by a rod attached to a metal holder. Neuronal recordings were performed using silicon probes from Cambridge Neurotech (16 contacts × 2 shanks per probe with 250 μm between shanks and 50-μm spacing between contact sites along each shank). Each shank had a thickness of 15 μm. Using a micromanipulator system (MX7600R, Siskiyou, Grants Pass, OR, USA), probes were positioned perpendicular to the pial surface based upon landmarks and stereotaxic coordinates and then inserted slowly into the brain through the intact dura mater to a depth of approximately 900 ± 50 μm at the deepest contact point. Neuronal data were acquired with an OmniPlex D Neural Data Acquisition System recording system (Plexon) at a sampling rate of 40 kHz (per channel) and 16-bit precision. Synchronization between the neural recordings and acoustic stimulus broadcasts was achieved with a TTL pulse output from the National Instrument card and recorded on one of the analog channels of the Plexon data acquisition system.