Mouse fibroblast cell line atcc 1658

Sodium salts of alginic acid, calcium chloride, ethylenediaminetetraacetic acid (EDTA), and sodium chloride were obtained from Sigma-Aldrich (St. Louis, MO). PVA (88% hydrolyzed, M.W. approximately 13 000–23 000) was bought from Acros Organics (New Jersey) and glycerol was bought from Fischer Chemicals (Fair Lawn, NJ). LB broth and LB Agar were obtained from Fisher Bioreagents (Fair Lawn, NJ). Dulbecco’s Modification of Eagle’s medium (DMEM) and trypsin-EDTA were purchased from Corning (Manassas, VA). The Cell Counting Kit-8 (CCK-8) was obtained from Sigma-Aldrich (St Louis, MO). The antibiotic penicillin-streptomycin (Pen-Strep) and fetal bovine serum (FBS) were purchased from Gibco-Life Technologies (Grand Island, NY 14072). l-Glutathione (reduced 98+ %) was purchased from Alfa Aesar (Ward Hill, MA). The bacterial strains Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 5538) as well as the mouse fibroblast cell line (ATCC 1658) were originally obtained from American Tissue Culture Collection (ATCC). Autoclaved phosphate buffered saline (PBS) was used for all in vitro experiments. The Culture-insert 2-well in μ-Dish (35 mm, high) used for cell migration experiment was purchased from ibidi (Martinsried, Germany).

Autoclaved phosphate-buffered saline (1× PBS) was used for all in vitro experiments. PCL (M_n = 80,000), gelatin from bovine skin, DAPI, hydrochloric acid, sodium nitrite, copper(II) chloride, l-ascorbic acid sodium salt, acetone, Cell Counting Kit-8 (CCK-8), and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) were purchased from Sigma-Aldrich (St. Louis, MO). l-Glutathione (reduced 98+%) was obtained from Alfa Aesar (Ward Hill, MA). Alexa Fluor 488 Phalloidin was purchased from Thermo Fisher Scientific (Waltham, MA). Dulbecco’s modified Eagle’s medium (DMEM) and trypsin–EDTA were purchased from Corning (Manassas, VA). The antibiotic penicillin–streptomycin (Pen–Strep) and fetal bovine serum were purchased from Gibco-Life Technologies (Grand Island NY 14072). The mouse fibroblast cell line (ATCC 1658) and Staphylococcus aureus (ATCC 5538) were originally obtained from American Tissue Culture Collection (ATCC).