Extracts of plant leaves were obtained as described by Talhaoui et al. [45 (link)], with some modifications. Freeze-dried leaves (250 mg) were ground to a powder and extracted twice via Ultra-Turrax IKA T18 basic, using 10 mL of MeOH/H2O (80/20 v/v). Afterwards, samples were placed in an ultrasonic liquid processor (Sonics & Materials, Inc., Newtown, CT, USA) for 10 min, and centrifuged at 4000 rpm for 10 min at 4 °C. After solvent evaporation, extracts were reconstituted with 4 mL of MeOH:H2O (50:50 v/v) and filtered through a 0.2 µm membrane (Minisart®, Sartorius Stedim Biotech CA, Goettingen, Germany).
Ultrasonic liquid processor
Manufactured by Sonics
Sourced in United States
The Ultrasonic Liquid Processor is a laboratory equipment designed to homogenize, emulsify, and disrupt samples through the application of high-frequency sound waves. It is capable of processing a wide range of liquid samples, including suspensions, emulsions, and solutions.
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Lab products found in correlation
2 protocols using ultrasonic liquid processor
1
Extraction and Analysis of Plant Metabolites
2
Immunoblotting Analysis of sCSF1R-HA-2A
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Immunoblotting was performed to detect the relative levels of sCSF1R-HA-2A in the brain, spinal cord, and cerebrospinal fluid. Tissues were weighed and minced, and then tissue homogenization buffer (PBS containing 0.04% bovine serum albumin, Complete Protease inhibitors (Roche)) was added at 5 volumes of the tissue weight. Tissues were homogenized by sonication (30 W, 3 × 15 sec) using a sonicator (Vibra-Cell Ultrasonic Liquid Processor, Sonics & Materials Inc, Newtown, CT). Homogenates were centrifuged at 10,000 ×g for 20 min at 4°C and the supernatants were collected.
Ten μl of supernatant from the brain, spinal cord (cervical and thoracic segments, lumbar and sacral segments), 5 μl of cerebrospinal fluid, and 0.5 μl of conditioned medium from pAAV/sCSF1R-transfected HEK293 cells were loaded into a 4 -12% gradient gel. The rest of the procedure was the same as that described above. Anti-HA antibody (1:5000) was used to detect the sCSF1R-HA-2A on the blot.
Ten μl of supernatant from the brain, spinal cord (cervical and thoracic segments, lumbar and sacral segments), 5 μl of cerebrospinal fluid, and 0.5 μl of conditioned medium from pAAV/sCSF1R-transfected HEK293 cells were loaded into a 4 -12% gradient gel. The rest of the procedure was the same as that described above. Anti-HA antibody (1:5000) was used to detect the sCSF1R-HA-2A on the blot.
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